Human HGF Protein, His Tag, premium grade

分子別名（Synonym）

HGF,HPTA,SF

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human HGF, His Tag, premium grade (HGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).

Predicted N-terminus: Gln 32

該產(chǎn)品是在我們嚴(yán)格的質(zhì)量控制體系下生產(chǎn)的，該體系包含一套全面的測試，包括無菌和內(nèi)毒素測試。在早期臨床前階段，產(chǎn)品性能經(jīng)過仔細(xì)驗(yàn)證和測試，以確保其與細(xì)胞培養(yǎng)用途或任何其他應(yīng)用的兼容性。當(dāng)準(zhǔn)備過渡到后期臨床階段時(shí)，我們還提供定制的GMP蛋白質(zhì)服務(wù)，以滿足您的需求。我們將與您合作，根據(jù)您的要求定制和開發(fā)GMP級(jí)產(chǎn)品，該產(chǎn)品也符合細(xì)胞療法細(xì)胞制造中使用的原材料和輔助材料的要求。

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

肝細(xì)胞生長因子（HGF）是一種旁分泌細(xì)胞生長、運(yùn)動(dòng)和形態(tài)發(fā)生因子。通過與受體酪氨酸激酶MET結(jié)合并促進(jìn)其二聚化來激活配體。肝細(xì)胞生長因子由間充質(zhì)細(xì)胞分泌，在主要來源于上皮的細(xì)胞上起多功能細(xì)胞因子的作用。其刺激有絲分裂、細(xì)胞運(yùn)動(dòng)和基質(zhì)侵襲的能力使其在血管生成、腫瘤發(fā)生和組織再生中起著核心作用。此外，HGF與多種癌癥有關(guān)，包括肺癌、胰腺癌、甲狀腺癌、結(jié)腸癌和乳腺癌。