Human TIGIT Protein, His Tag, active dimer (MALS verified)
分子別名(Synonym)
TIGIT,VSIG9,VSTM3
表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)
Human TIGIT, His Tag (TIT-H52H5) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).
Predicted N-terminus: Met 22
蛋白結(jié)構(gòu)(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.8 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>90% as determined by SDS-PAGE.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
具有Ig和ITIM結(jié)構(gòu)域的T細(xì)胞免疫受體(TIGIT)也稱為V-set和含免疫球蛋白結(jié)構(gòu)域的蛋白9(VSIG9)、V-set和跨膜結(jié)構(gòu)域的蛋白質(zhì)3(VSTM3),屬于單程I型膜蛋白,含有免疫球蛋白可變結(jié)構(gòu)域、跨膜結(jié)構(gòu)區(qū)和基于免疫受體酪氨酸的抑制基序(ITIM)。TIGIT在外周記憶和調(diào)節(jié)性CD4+T細(xì)胞和NK細(xì)胞上以低水平表達(dá),并在這些細(xì)胞激活后上調(diào)(在蛋白質(zhì)水平)。TIGIT以高親和力結(jié)合脊髓灰質(zhì)炎病毒受體(PVR),導(dǎo)致IL10分泌增加和IL12B分泌減少,并通過促進(jìn)成熟免疫調(diào)節(jié)樹突狀細(xì)胞的產(chǎn)生來抑制T細(xì)胞活化。
關(guān)鍵字: TIGIT;TIGIT蛋白;TIGIT重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。