RN08-EASYspin 細菌RNA快速提取試劑盒
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產(chǎn)品介紹:
獨特的裂解液迅速裂解細胞和滅活細胞RNA酶,然后用乙醇調(diào)節(jié)結(jié)合條件后,RNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜, 再通過一系列快速的漂洗-離心的步驟, 去蛋白液和漂洗液將細胞代謝物,蛋白等雜質(zhì)去除, z后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點:
1.離心吸附柱內(nèi)硅基質(zhì)膜全部采用進口世界有名公司特制吸附膜,柱與柱之間吸附量差異極小,可重復性好??朔藝a(chǎn)試劑盒膜質(zhì)量不穩(wěn)定的弊端。2.不需要使用有毒的苯酚,氯仿等試劑,也不需要乙醇沉淀等步驟。3.快速,簡捷,單個樣品操作一般可在30分鐘內(nèi)完成。4.多次柱漂洗確保高純度,OD260/OD280典型的比值達1.9~2.0,基本無DNA殘留,可用于RT-PCR,Northern-blot和各種實驗。
使用該產(chǎn)品發(fā)表的部分文章:http://www.aidlab.cn/news-show.asp?id=192
EASYspin 細菌RNA提取試劑盒發(fā)表文章
Cloning and characterization of bagB and bagC, two co-transcribed genes involved in bagremycin biosynthesis in Streptomyces sp. Tü 4128. Ann Microbiol 2013,63(1), : 167–172
EASYspin Plus細菌RNA提取試劑盒發(fā)表文章
Relative significances of pH and substrate starch level to roles of Streptococcus bovis S1 in rumen acidosis. AMB Express 2016, 6:80 DOI: 10.1186/s13568-016-0248-2
Design of Ribosome Binding Sites in Streptomyces coelicolor.Current Proteomics 2017, 14(4): 287-292
Antibacterial activity of gallic acid against Shigella flexneri and its effect on biofilm formation by repressing mdoH gene expression. Food Control 2018, 94: 147-154
Study on the drug resistance of clinical strain of acinetobacter baumannii mediated by TEM-1 beta-lactamases and porin CarO. Tianjin Med J, 2018, 46(3): 246-250
Contribution of the colicin receptor CirA to biofilm formation, antibotic resistance, and pathogenicity of Salmonella Enteritidis. J Basic Microbiol. 2019, 1–10. https://doi.org/10.1002/jobm.201900418
FadR1, a pathway-specific activator of fidaxomicin biosynthesis in Actinoplanes deccanensis Yp-1. Applied Microbiology and Biotechnology 2019, https://doi.org/10.1007/s00253-019-09949-y
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