Human FAP Protein, His Tag, active dimer (MALS verified)
分子別名（Synonym）
FAP,FAPalpha,SIMP,Seprase,APCE
表達區(qū)間及表達系統(tǒng)（Source）
Human FAP, His Tag (FAP-H5244) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).
Predicted N-terminus: His
蛋白結構（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 86.9 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
內毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
FAP（也稱為seprase）是一種II型跨膜絲氨酸蛋白酶。質膜和可溶性形式都表現出脯氨酸切割后的內肽酶活性，對Ala/Ser-Gly-Pro-Ser/Asn/Ala共有序列有顯著的偏好。還可降解明膠，使I型膠原熱變性。還具有二肽基肽酶活性，優(yōu)選Ala-Pro、Ile-Pro、Gly-Pro、Arg-Pro和Pro-Pro。質膜形式與DPP4、PLAUR或整合素結合，參與細胞外基質（ECM）的細胞周蛋白水解，從而促進細胞通過ECM的粘附、遷移和侵襲。通過降解蛋白聚糖短肽促進神經膠質瘤細胞通過腦實質的侵襲。通過以絲氨酸蛋白酶活性獨立的方式調節(jié)細胞增殖和存活，在黑素細胞中充當腫瘤抑制因子。
關鍵字： FAP;FAP蛋白;FAP重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產業(yè)領域提供關鍵生物試劑產品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。