Human B7-H4 Protein, His Tag (MALS verified)

分子別名（Synonym）

B7-H4,VTCN1,B7S1,B7h.5

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human B7-H4, His Tag (B74-H5222) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # NP_078902).

Predicted N-terminus: Phe 29

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.4 kDa. The protein migrates as 39-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

無(wú)菌（Sterility）

Negative

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

含V集結(jié)構(gòu)域的T細(xì)胞活化抑制劑1（VTCN1）也稱(chēng)為免疫共刺激蛋白B7-H4、蛋白B7S1、T細(xì)胞共刺激分子B7x、B7H4，屬于免疫球蛋白超家族和BTN/MOG家族。VTCN1包含兩個(gè)Ig樣V型（免疫球蛋白樣）結(jié)構(gòu)域。VTCN1的表達(dá)被IL6和IL10上調(diào)，并被抗原呈遞細(xì)胞（APC）上的GM-CSF和IL4抑制。VTCN1/B7-H4通過(guò)抑制T細(xì)胞的活化、增殖、細(xì)胞因子的產(chǎn)生和細(xì)胞毒性的發(fā)展來(lái)負(fù)調(diào)控T細(xì)胞介導(dǎo)的免疫反應(yīng)。VTCN1參與促進(jìn)上皮細(xì)胞轉(zhuǎn)化。