Human HGFR / c-MET Protein, His Tag

分子別名（Synonym）

MET,AUTS9,HGFR,RCCP2,c-Met

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human HGF R, His Tag (MET-H5227) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932 (Accession # AAI30421.1).

Predicted N-terminus: Glu 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa and 70.2 kDa. The protein migrates as 40-45 kDa and 85-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

肝細(xì)胞生長(zhǎng)因子受體（HGFR）也被稱為間質(zhì)-上皮轉(zhuǎn)化因子（MET），c-MET，是一種糖基化受體酪氨酸激酶，在上皮形態(tài)發(fā)生和癌癥發(fā)展中起著核心作用。HGFR蛋白具有酪氨酸激酶活性。初級(jí)單鏈前體蛋白經(jīng)翻譯后切割產(chǎn)生α和β亞基，二硫鍵連接形成成熟受體。HGFR通常由上皮來(lái)源的細(xì)胞表達(dá)，而HGF的表達(dá)僅限于間充質(zhì)來(lái)源的細(xì)胞。在HGF刺激下，HGFR誘導(dǎo)幾種生物反應(yīng)，共同產(chǎn)生一種稱為侵入性生長(zhǎng)的程序。癌癥中HGFR的異常激活與預(yù)后不良相關(guān)，其中異常激活的HGFR觸發(fā)腫瘤生長(zhǎng)、為腫瘤提供營(yíng)養(yǎng)的新血管形成（血管生成）以及癌癥擴(kuò)散到其他器官（轉(zhuǎn)移）。HGFR在許多類型的人類惡性腫瘤中失調(diào)，包括腎癌、肝癌、胃癌、乳腺癌和腦癌。正常情況下，只有干細(xì)胞和祖細(xì)胞表達(dá)HGFR，然而，癌癥干細(xì)胞被認(rèn)為劫持了正常干細(xì)胞表達(dá)HGFL的能力，從而成為癌癥持續(xù)存在并擴(kuò)散到身體其他部位的原因。HGFR基因的各種突變與乳頭狀腎癌有關(guān)。HGFR介導(dǎo)一種稱為侵襲性生長(zhǎng)的復(fù)雜程序。HGFR的激活觸發(fā)有絲分裂和形態(tài)發(fā)生。