Human Glypican 3 / GPC3 Protein, His Tag, premium grade

分子別名（Synonym）

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Glypican 3, His Tag, premium grade (GP3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Predicted N-terminus: Gln 25 & Ser 359

該產(chǎn)品是在我們嚴(yán)格的質(zhì)量控制體系下生產(chǎn)的，該體系包括一系列全面的測試，包括無菌和內(nèi)毒素測試。在臨床前早期階段，產(chǎn)品性能經(jīng)過仔細(xì)驗(yàn)證和測試，可用于細(xì)胞培養(yǎng)或任何其他應(yīng)用。當(dāng)準(zhǔn)備進(jìn)入后期臨床階段時(shí)，我們還提供定制GMP蛋白質(zhì)服務(wù)，以滿足您的需求。我們將與您合作，根據(jù)您的要求定制和開發(fā)GMP級(jí)產(chǎn)品，該產(chǎn)品也符合細(xì)胞療法細(xì)胞制造中使用的原材料和輔助材料的要求。

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively. The protein migrates as 30 kDa, 40 kDa and 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Glypican-3（GPC3）也稱為腸蛋白OCI-5，GTR2-2，MXR7，屬于磷脂酰肌醇蛋白聚糖家族。磷脂酰肌醇蛋白聚糖3/GPC-3在肺，肝和腎中高度表達(dá)。磷脂酰肌醇蛋白聚糖-3抑制DPP4的二肽基肽酶活性。磷脂酰肌醇蛋白聚糖-3可能參與抑制/調(diào)節(jié)主要中胚層組織和器官的生長，也可能在調(diào)節(jié)IGF2與其受體的相互作用中發(fā)揮作用，從而調(diào)節(jié)其功能。