Human EGF R Protein, His Tag (MALS verified)

分子別名（Synonym）

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human EGF R, His Tag (EGR-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).

Predicted N-terminus: Leu 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 70.5 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

表皮生長(zhǎng)因子受體（EGFR；ErbB-1；人類(lèi)中的HER1）是細(xì)胞外蛋白配體表皮生長(zhǎng)因子家族（EGF家族）成員的細(xì)胞表面受體。表皮生長(zhǎng)因子受體是ErbB受體家族的成員，該家族由四種密切相關(guān)的受體酪氨酸激酶組成：EGFR（ErbB-1）、HER2/c-neu（ErbB-2）、Her 3（ErbB-3）和Her 4（ErbB-4）。影響EGFR表達(dá)或活性的突變可能導(dǎo)致癌癥。