IL-8產(chǎn)品信息
英文名稱：Interleukin-8
中文名稱：白細(xì)胞介素-8
靶點別稱：CXCL8,GCP1,IL-8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1
物種：Human / Cynomolgus
屬性：Protein / Kits
標(biāo)記：Biotin-labeled / Unconjugated
IL-8 Protein
內(nèi)毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
無菌（Sterility）
The sterility testing was performed by membrane filtration method.
支原體（Mycoplasma）
Negative.
純度（Purity）
>90% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.
IL-8 ELISA Kit
背景（Background）
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-8 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
應(yīng)用說明（Application）
The kit is developed for quantitative detection of IL-8 in human serum and cell culture supernates.
It is for research use only.
原理（Assay Principles）
This assay kit is used to measure the concentrations of human Interleukin 8 (IL-8) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-8 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-8 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存儲（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
IL-8分子背景
白細(xì)胞介素8（IL8或趨化因子（C-X-C基序）配體8，CXCL8）是由巨噬細(xì)胞和其他細(xì)胞類型（如上皮細(xì)胞、氣道平滑肌細(xì)胞和內(nèi)皮細(xì)胞）產(chǎn)生的趨化因子。表面膜上有許多能夠結(jié)合IL-8的受體，最常被研究的類型是G蛋白偶聯(lián)的蛇形受體CXCR1和CXCR2。兩種受體對IL-8的表達和親和力不同（CXCR1＞CXCR2）。與IL-8（1-77）相比，IL-8（6-77）對中性粒細(xì)胞活化的活性高出5-10倍。
IL-8用戶評價
關(guān)鍵字： IL-8;IL-8蛋白;IL-8細(xì)胞因子;IL-8試劑盒;白介素8;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。