熒光
λex?504?nm; λem?524?nm in 0.1 M Tris pH 8.0 (esterase)
一般描述
2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.
應用
2′,7′-二氯熒光素二乙酸酯可用作未衍生化熒光素的優(yōu)良氣相替代物，并可用作2′,7′-二氯熒光素二乙酸酯測定的組分/底物，以定量活性氧物質 (ROS)。
Sigma-Aldrich西格瑪奧德里奇(上海)貿易有限公司
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