Rabbit Anti-phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody
DATASHEET
Host:Rabbit
Target Protein:phospho-ERK1 + 2 (Thr183/Tyr185)
IR:Immunogen Range:FL(p-T)E(p-Y)V
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5595
Swiss Prot:P63085
Source:KLH conjugated Synthesised phosphopeptide derived from mouse ERK1 around the phosphorylation site of Thr183/Tyr185:FL(p-T)E(p-Y)V
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]
Size:50ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
Rabbit
Guinea Pig
本品僅供科研使用。不能用于人和動(dòng)物治療等其它臨床診斷用！
關(guān)鍵字： phospho-ERK1 + 2 (Th;ERK1 + ERK2 (phospho;phospho-ERK1/MAPK-1/;ERK 1;ERK 2;ERK1;p44MAPK;PRKM1;
上海滬震實(shí)業(yè)有限公司是一家集研發(fā)、生產(chǎn)和銷售于一體的生命科學(xué)實(shí)驗(yàn)室產(chǎn)品生物科技企業(yè)。主營(yíng)產(chǎn)品：試劑盒，ELISA試劑盒，抗體，重組蛋白，血清，胎牛血清，細(xì)胞，原代細(xì)胞 細(xì)胞培養(yǎng)試劑，常用生化試劑。