Host:Rabbit

Target Protein:phospho-Cyclin E1 (Thr77)

IR:Immunogen Range:IP(p-T)PD

Clonality:Monoclonal

Isotype:IgG

Entrez Gene:898

Swiss Prot:P24864

Source:KLH conjugated Synthesised phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77:IP(p-T)PD 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Size:50ul

Concentration:1mg/ml

Applications:WB(1:200-500)
IHC-P(1:50-200)
IHC-F(1:50-200)
ICC(1:50-200)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat