產(chǎn)品詳情
中文名稱:HRP快速標(biāo)記試劑盒 | 英文名稱:HRP Labeling Kit |
品牌: ECALBIO | 產(chǎn)品類別: Reagent |
數(shù)量: 1000 | 保質(zhì)期: 3年 |
2025-01-03
HRP快速標(biāo)記試劑盒
HRP Labeling Kit
1盒/3000RMB
3000
ECALBIO
Reagent
1. 原理與應(yīng)用
高碘酸鈉氧化法活化辣根過(guò)氧化物酶(HRP)的糖基,經(jīng)過(guò)活化的HRP帶有醛基可以與待標(biāo)記物的游離氨基結(jié)合,發(fā)生希夫堿反應(yīng)(Schiff's base reaction)。本試劑盒適用于帶有游離氨基的物質(zhì)標(biāo)記,如:帶有游離氨基(伯胺)的抗體、蛋白或者小分子化合物。
2. 試劑盒組成
預(yù)活化凍干HRP | 5x1mg |
標(biāo)記啟動(dòng)液 | 2mL |
反應(yīng)終止液干粉 | 1管可配1mL |
標(biāo)記物保存液 | 5mL |
備用透析袋(MD6,截留量12KD) | 10X5CM |
說(shuō)明書 | 1張 |
3. 緩沖液配置
(1) 0.01M CB,PH:9.6緩沖液配制:取NaHCO3, 0.67g和Na2CO3, 0.21g加水到1L溶解,不用調(diào)節(jié)PH。
(2) 0.067M PBS, PH:7.0緩沖液配制:取NaH2PO4.2H2O,5.35g和Na2HPO4.12H2O, 11.7g以及NaCL,9g加水1L溶解,添加0.35g NaOH再次混勻,不用調(diào)節(jié)PH。
4. 使用方法
(1) 首先去除待標(biāo)記抗體或蛋白中的疊氮鈉、以及含有氨基的緩沖成分物質(zhì),比如甘氨酸、Tris 等,還有EDTA等物質(zhì),可采用透析和超濾的辦法置換緩沖液,以去除干擾物質(zhì)達(dá)到標(biāo)記效果!透析或超濾緩沖液首選0.01M CB,PH:9.6緩沖液,若實(shí)驗(yàn)室沒(méi)有碳酸鹽試劑再采用0.01M PBS(PH:7.0-7.4均可)透析或超濾置換緩沖液,最后調(diào)整抗體或蛋白的濃度到2MG/ML。如果抗體和蛋白不含上述這些干擾物質(zhì)可以直接用上述PBS或CB緩沖液調(diào)整濃度到2MG/ML備用。
(2) 從-20℃取出HRP標(biāo)記試劑盒,室溫條件25-30℃溫箱平衡30分鐘左右,使各組分充分解凍后混勻。
(3) 取500uL抗體或蛋白(約1mg),加入一只預(yù)活化HRP(1mg)凍干粉中,用移液槍反復(fù)吹打幾次混勻HRP和抗體,混勻后加入HRP標(biāo)記啟動(dòng)液80ul繼續(xù)混勻數(shù)次,避免產(chǎn)生氣泡。最后室溫避光或25-30℃溫箱偶聯(lián)反應(yīng)2-3小時(shí),若遇快要下班也可放4℃反應(yīng)24小時(shí)左右(效果一樣或者更好不必?fù)?dān)心)。
(4) 偶聯(lián)結(jié)束將反應(yīng)終止液粉管中加入1mL去離子水混勻,取50ul加入3所述反應(yīng)液中,充分混勻,室溫放置1小時(shí)或者4℃ 2小時(shí)。
(5) 終止完成后,可直接加入等體積的標(biāo)記物保存液,充分混勻,置于-20℃保存。如果長(zhǎng)期保存,建議先用0.067M PBS PH=7.0透析或超濾置換緩沖液后再加標(biāo)記物保存液??梢员4?/span>3年以上。
關(guān)鍵字: HRP標(biāo)記試劑盒;HRP快速標(biāo)記;HRP標(biāo)記;
公司簡(jiǎn)介
Ecalbio Co.; Ltd specialize in developing reagents of immunassay technology and biological systems with higher accuracy and reliability for results of the highest quality.
Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.
Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.
Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.
Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
Government Laboratories
Food industry Laboratories
Institutes
Veterinary Clinics
Pharmaceutical Industry.
Ecalbio welcomes potential partners and distributors to explore business relationships with all customer in the worldwide, focused on scie-tech, integrity, quality and service for the customers.
成立日期 |
2019-09-19
(6年)
|
注冊(cè)資本 |
500萬(wàn)
|
員工人數(shù) |
50-100人 |
年?duì)I業(yè)額 |
¥ 1000萬(wàn)-5000萬(wàn) |
主營(yíng)行業(yè) |
抗體,分子生物學(xué),診斷試劑,綠色化學(xué)產(chǎn)品,診斷試劑 |
經(jīng)營(yíng)模式 |
貿(mào)易,工廠,試劑,定制 |