產(chǎn)品詳情
純度：
Greater than 93% as determined by SDS-PAGE.
內(nèi)毒素：
Less than 1.0 EU/ug as determined by LAL method.
生物活性：
①M(fèi)easured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody(CSB-RA156386A0HU), the EC50 is 2.353-3.232 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized CD40(CSB-MP004936HU1) at 2 μg/ml can bind CD40L, the EC50 is 3.112-3.858 ng/ml. ③Human CD40 protein hFc tag (CSB-MP004936HU1) captured on COOH chip can bind Human CD40L protein hFc and Flag tag (CSB-MP004937HU3) with an affinity constant of 2.06 nM as detected by LSPR Assay.
基因名：
CD40LG
Uniprot No.：
P29965
別名：
(CD40-L)(T-cell antigen Gp39)(NF-related activation protein)(TRAP)(Tumor necrosis factor ligand superfamily member 5)(CD154)(sCD40L)
分子結(jié)構(gòu)：
種屬：
Homo sapiens (Human)
蛋白長(zhǎng)度：
Partial
來(lái)源：
Mammalian cell
分子量：
44.5 kDa
表達(dá)區(qū)域：
113-261aa
氨基酸序列
MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
蛋白標(biāo)簽：
N-terminal hFc-Flag-tagged
產(chǎn)品提供形式：
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
復(fù)溶：
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：
3-7 business days
注意事項(xiàng)：
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：
Human CD40 ligand (CD40LG) amino acid Met13-Leu261, with an N-terminal hFc-Flag-tag as well as an N-terminal linker, was expressed in mammalian cells. The product is the recombinant human CD40LG protein. The SDS-PAGE analysis determined its purity greater than 90% and showed a molecular weight band of about 45 kDa on the gel. It contains low levels of endotoxin, less than 1.0 EU/ug measured by the LAL method. Functional ELISA and LSPR assay of this CD40LG protein showed that it is an active protein. This protein is in stock so that it will reach your lab bench faster.
CD40LG, also known as CD154, is a ligand for the CD40. It is preferentially expressed by activated CD4+ T cells as well as activated B cells and platelets. The interaction of CD40 and CD40LG plays an essential role in immune regulation and homeostasis. The CD40-CD40L system is also involved in the pathophysiology of several diseases, including autoimmune diseases, atherothrombosis, respiratory disease, and cancer.
艾普蒂生物科技（ipodix）多年來(lái)致力于重組蛋白表達(dá)與純化的相關(guān)研究，每年可完成上百組蛋白的表達(dá)與純化服務(wù)，擁有完整的蛋白純化體系，可根據(jù)客戶的不同需求提供多元化的蛋白純化方案。我們可為您提供定制的蛋白純化服務(wù)包括，有/無(wú)標(biāo)簽重組蛋白，天然蛋白的純化分離等。根據(jù)蛋白性質(zhì)設(shè)計(jì)親和純化、離子交換、分子篩、疏水等多種蛋白純化實(shí)驗(yàn)方案，獲得的高純度蛋白質(zhì)，可根據(jù)客戶需求一對(duì)一進(jìn)行個(gè)性化方案定制，能夠滿足蛋白質(zhì)折疊、重結(jié)晶等一系列精細(xì)實(shí)驗(yàn)要求。
重組蛋白純化：
重組蛋白分離純化一般分為三種：含標(biāo)簽的重組蛋白純化、不含標(biāo)簽的重組蛋白純化以及包涵體變性與復(fù)性純化。
含標(biāo)簽的重組蛋白純化：組氨酸標(biāo)簽（His-tag）是使用范圍較為廣泛的一種蛋白純化標(biāo)簽，其純化的原理是組氨酸殘基上的咪唑基團(tuán)可與層析介質(zhì)中的金屬離子螯合，再通過(guò)提高緩沖液中咪唑濃度進(jìn)行競(jìng)爭(zhēng)性洗脫達(dá)到純化效果，這種純化標(biāo)簽的有點(diǎn)在于His-Tag的分子質(zhì)量非常小且?guī)缀醪粫?huì)對(duì)目的蛋白本身的生物學(xué)功能及特性產(chǎn)生影響，因此帶有His-Tag的蛋白純化后可直接作為抗原免疫動(dòng)物制備抗體并不會(huì)影響抗體的特異性。艾普蒂生物還提供其他多種類型標(biāo)簽的蛋白純化服務(wù)，如GST-Tag、SUMO-Tag、MBP-Tag等。
圖1 6xHis標(biāo)簽和Ni-NTA相鄰殘基之間的相互作用
不含標(biāo)簽的重組蛋白純化：不含純化標(biāo)簽的重組蛋白我們會(huì)根據(jù)客戶的樣品及需求定制方案，一般會(huì)采用抗體親和純化方法，利用靶向配體能與目的蛋白特異性結(jié)合的特性將目的蛋白分離出來(lái)；或選用分子篩進(jìn)行分離純化。
包涵體變性與復(fù)性純化：將以包涵體形式存在的蛋白進(jìn)行變復(fù)性操作，根據(jù)蛋白質(zhì)不同的性質(zhì)，可以選擇尿素、鹽酸胍、去垢劑、極端pH等，以保證程度獲得單體狀態(tài)的變性蛋白。
艾普蒂生物提供的蛋白分離純化流程：
步驟 | 服務(wù)內(nèi)容 | 周期 |
Step1 純化前檢測(cè)與預(yù)實(shí)驗(yàn) | SDS-PAGE檢測(cè)蛋白情況； 小體積純化與檢測(cè) | 1-2周 |
Step2 蛋白純化及檢測(cè) | 大量純化； SDS-PAGE； WB檢測(cè)（視項(xiàng)目而定）； 濃度檢測(cè) | 1-2周 |
交付 | 成品蛋白，純化報(bào)告，圖片數(shù)據(jù)； |
服務(wù)優(yōu)勢(shì)：
--設(shè)備一流，純化方式多元化，適合不同性質(zhì)的重組蛋白；
--專業(yè)技術(shù)團(tuán)隊(duì)為您量身定制純化方案，獲得能夠滿?下游實(shí)驗(yàn)的?純度蛋?；
--提供全面的蛋白純化分析報(bào)告；
關(guān)鍵字： CD40LG 蛋白;人CD40LG 重組蛋白;CD40LG;
湖北艾普蒂生物工程有限公司（簡(jiǎn)稱：艾普蒂）由在國(guó)內(nèi)科研試劑領(lǐng)域有著十幾年從業(yè)經(jīng)驗(yàn)的專業(yè)技術(shù)團(tuán)隊(duì)和企業(yè)管理團(tuán)隊(duì)組建而成，專門(mén)從事以抗體、蛋白、細(xì)胞、化學(xué)品為核心的試劑產(chǎn)品研發(fā)與銷售。
我們長(zhǎng)期聚焦于生命科學(xué)、生物醫(yī)藥、醫(yī)療診斷、分析檢測(cè)等領(lǐng)域，專注于為生命科學(xué)和生物技術(shù)領(lǐng)域的客戶提供優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務(wù)。提供生命科學(xué) 試劑、供應(yīng)鏈服務(wù)等，為更多的生命科學(xué)科研工作者提供 專業(yè)、全面、安全、快捷的產(chǎn)品和服務(wù)。產(chǎn)品覆蓋 基因、蛋白、細(xì)胞、組織、動(dòng)物等水平的各種研究。
致力于成長(zhǎng)為我國(guó)重要的生命科學(xué)試劑和成果轉(zhuǎn)化一體化的領(lǐng)導(dǎo)者、致力于成為中國(guó)大型的科研試劑產(chǎn)品供應(yīng)商之一。