別名：ApoL related ligand TALL 1 ELISA Kit; B cell Activating Factor ELISA Kit; B lymphocyte stimulator ELISA Kit; B-cell-activating factor ELISA Kit; BAFF ELISA Kit; BLyS ELISA Kit; CD 257 ELISA Kit; CD257 ELISA Kit; CD257 antigen ELISA Kit; Delta BAFF ELISA Kit; Dendritic cell derived TNF like molecule ELISA Kit; Dendritic cell-derived TNF-like molecule ELISA Kit; DTL ELISA Kit; DTL precursor ELISA Kit; PRO738 ELISA Kit; soluble form ELISA Kit; TALL 1 ELISA Kit; TALL-1 ELISA Kit; TALL1 ELISA Kit; THANK ELISA Kit; TN13B_HUMAN ELISA Kit; TNF and APOL related leukocyte expressed ligand 1 ELISA Kit; TNF homolog that activates apoptosis ELISA Kit; TNF homolog that activates apoptosis NKFB and JNK. ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 1 ELISA Kit; TNFSF13B ELISA Kit; TNFSF20 ELISA Kit; TNLG7A ELISA Kit; Tumor necrosis factor (ligand) superfamily member 13b ELISA Kit; Tumor necrosis factor ligand 7A ELISA Kit; Tumor necrosis factor ligand superfamily member 13b ELISA Kit; Tumor necrosis factor ligand superfamily member 20 ELISA Kit; Tumor necrosis factor like protein ZTNF4 ELISA Kit; Tumor necrosis factor superfamily member 13B ELISA Kit; UNQ401 ELISA Kit; ZTNF4 ELISA Kit

縮寫：TNFSF13B

Uniprot No.：Q9Y275

種屬：Homo sapiens (Human)

樣本類型：serum, plasma, tissue homogenates

檢測(cè)范圍：

0.312 ng/mL-20 ng/mL

靈敏度： 0.078 ng/mL

反應(yīng)時(shí)間：1-5h

樣本體積：50-100ul

檢測(cè)波長：450 nm

研究領(lǐng)域：Immunology

測(cè)定原理：quantitative

測(cè)定方法：Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BAFF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

　 Sample Serum(n=4)

1:1 Average % 88

Range % 82-95

1:2 Average % 90

Range % 85-96

1:4 Average % 93

Range % 90-95

1:8 Average % 92

Range % 88-96

回收率：

The recovery of human BAFF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type Average % Recovery Range

Serum (n=5) 92 85-99

EDTA plasma (n=4) 90 87-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected

20 2.912 2.953 2.933 2.819

10 2.142 2.175 2.159 2.045

5 1.295 1.312 1.304 1.190

2.5 0.727 0.695 0.711 0.597

1.25 0.426 0.443 0.435 0.321

0.625 0.297 0.312 0.305 0.191

0.312 0.247 0.261 0.254 0.140

0 0.112 0.115 0.114 　

注意事項(xiàng)：

1、試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開封后如未用完，板條應(yīng)裝入密封袋中保存

2、濃洗滌液可能會(huì)有結(jié)晶析出，稀釋時(shí)可在水浴中加溫助溶，洗滌時(shí)不影響結(jié)果

3、各步加樣均應(yīng)使用加樣器，并經(jīng)常校對(duì)其準(zhǔn)確性，以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣

4、請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值)，請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定計(jì)算時(shí)請(qǐng)最后乘以總稀釋倍數(shù)(xnx5)

5、封板膜只限一次性使用，以避免交叉污染

6、底物請(qǐng)避光保存。

7、嚴(yán)格按照說明書的操作進(jìn)行，試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn)

8、所有樣品，洗滌液和各種廢棄物都應(yīng)按傳染物處理

9、.本試劑不同批號(hào)組分不得混用。

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