中文名稱
英文名字
Recombinant Human Glycogen Phosphorylase
供應(yīng)商
ProSpec
產(chǎn)品貨號(hào)
ENZ-282
背景資料
Glycogen phosphorylase is one of the phosphorylaseenzymes(EC2.4.1.1). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule, and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism, it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase.Glycogen phosphorylase can only act on linearchainsof glycogen(a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch(which are exceedingly common in glycogen). In these situations, a debranching enzymeis necessary, which will straighten out the chain in that area. Additionally, an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done, glycogen phosphorylase can continue.An insulinstimulated enzyme known as phosphoprotein phosphatase(PP-1) inactivates glycogen phosphorylase to prevent glycogen break up.GPBB - a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. GPBB can also be a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.
序列
該【GPBB】重組人糖原磷酸化酶Recombinant Human Glycogen Phosphorylase是Prospec品牌 產(chǎn)品,具體的蛋白序列可查看提供的英文版本產(chǎn)品說(shuō)明書(shū)。
來(lái)源宿主
大腸桿菌中重組表達(dá)
溶解建議
提供的人糖原磷酸化酶(GPBB)產(chǎn)品為過(guò)濾滅菌的濃度為0.8 mg/ml無(wú)色溶液;其中每毫克人糖原磷酸化酶(GPBB)產(chǎn)品溶解于50%甘油溶液中.
保存建議
人糖原磷酸化酶(GPBB)溶液可以在10℃保存7天;如果想延長(zhǎng)保存時(shí)間,建議分裝后置于-18℃凍存.請(qǐng)避免反復(fù)凍融.
其他
ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司,具有20多年的生產(chǎn)經(jīng)驗(yàn),專注于蛋白(重組及合成)生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括:干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長(zhǎng)因子、瘦素、干細(xì)胞因子、催乳素、表皮生長(zhǎng)因子、胰島素樣生長(zhǎng)因子、神經(jīng)生長(zhǎng)因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白,價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白,而不含有標(biāo)簽蛋白??紤]到大部分研究者希望能更靈活地配制蛋白溶液,ProSpec-Tany絕大部分產(chǎn)品沒(méi)有添加保護(hù)劑或鹽,這就對(duì)工藝提出了更高的要求。同時(shí),ProSpec-Tany絕大部分產(chǎn)品為凍干粉,因此易于運(yùn)輸和保存。
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