中文名稱

細胞滲透性 Rho 抑制劑（基于C3轉移酶）

英文名字

Cell Permeable Rho Inhibitor (C3 Trans based))

供應商

Cytoskeleton

產品貨號

CT04-A

背景資料

該細胞滲透性 Rho 抑制劑（基于C3轉移酶）產品是Cytoskeleton品牌原裝進口，Cytoskeleton品牌是細胞骨架研究專家，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產。 

產品描述

This product consists of highly purified C3 Transferase (Cat. # CT03) covalently linked to a proprietary cell penetrating moiety via a disulfide bond.  The cell penetrating moiety allows rapid and efficient transport through the plasma membrane.  Once in the cytosol, the cell penetrating moiety is released, thereby allowing C3 Transferase to freely diffuse intracellularly and inactive RhoA, RhoB, and RhoC, but not related GTPases such as Cdc42 or Rac1.The Exoenzyme C3 Transferase from Clostridium botulinum is commonly used to selectively inactivate the GTPases RhoA, RhoB, and RhoC, both in vivo and in vitro.  C3 Transferase inhibits Rho proteins by ADP-ribosylation on asparagine 41 in the effector binding domain of the GTPase.  A major limitation of C3 Transferase for use in in vivoapplications is that this protein is only slightly cell permeable.  Consequently, overnight incubations with C3 Transferase at concentrations as high as 100 μg/ml are often necessary to inactivate Rho proteins in cultured cells.  Under these conditions, the long incubation period and large amount of C3 Transferase that are required can be disruptive to basic cellular functions and economically burdensome.  Cytoskeleton, Inc. has overcome these problems by providing a cell permeable form or C3 Transferase that can efficiently inactivate cellular Rho proteins in as little as 2 h. 

產品形式

該細胞滲透性 Rho 抑制劑（基于C3轉移酶）產品是Cytoskeleton品牌的原裝進口產品，具體的產品形式查看英文產品說明書。

保存建議

廠家推薦常溫運輸，收到產品后建議保存于4°C。

其他

Cytoskeleton公司成立于1993年，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產。公司提供藥物篩選、信號轉導、蛋白質轉錄后修飾（PTM）、細胞骨架研究相關的系列試劑盒和產品，尤其以細胞骨架相關研究見長，既能滿足于樣品較少的科學研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動蛋白，小G蛋白，GAPs，GEFs等現有產品的藥物篩選服務。