中文名稱

唾液外泌體純化試劑盒（提取試劑盒）

英文名字

Saliva Exosome Purification Kit

供應(yīng)商

Norgen Biotek

產(chǎn)品貨號

NGB-65300

背景資料

唾液外泌體純化試劑盒（提取試劑盒）產(chǎn)品的背景資料詳情查看Norgen Biotek品牌提供的產(chǎn)品說明書

產(chǎn)品描述

Exosomes are 40 - 150 nm membrane vesicles which are secreted by most cell types. Exosomes can be found in plasma, serum, saliva, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes and other extracellular vesicles (EVs) which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNA may serve as biomarkers for various diseases including cancer. Another subset of RNA that is found in saliva is the free-circulating RNA (fc-RNA). These fc-RNA are usually protein-bound RNA that are leaked from cells either during apoptosis or necrosis. As the RNA molecules encapsulated within exosomes or bound to proteins (fc-RNA) are protected from degradation by RNAses, they can be efficiently recovered from biological fluids such as saliva. In general, these two RNA groups contain valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status.Norgen’s Saliva Exosome Purification Kit allows for the purification of saliva exosomes from 500 μL to 2 mL of fresh saliva samples or saliva samples collected using Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400). This kit also allows for the purification of intact extracellular vesicles (EVs) from different saliva sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-bound proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

產(chǎn)品特點

Purification and enrichment of intact saliva exosomes;Versatile sample input ranging from 0.5 mL to 2 mL;Process fresh saliva or saliva collected with Norgen's Saliva;Exosome Collection and Preservation Kit (Cat 65400).;No time-consuming ultracentrifugation, filtration nor special syringes required;No precipitation reagents or overnight incubation required;No protease treatment required;Purify pure exosomes that are free from RNA-binding proteins;Purified exosomes can be analyzed using NanoSight? or Electron Microscopy for assessing the approximate exosome size range and concentration;RNA can be isolated from the purified exosomes using Norgen's Exosome RNA Purification kit (Cat. 58000).

保存建議

溶液密封室溫(15-25°C)保存，未開封至少保存2年。

其他

Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術(shù)公司，基于專利的碳化硅膜純化技術(shù)和無苯酚專利配方，生產(chǎn)高品質(zhì)的核酸和蛋白質(zhì)純化及富集產(chǎn)品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發(fā)的科技創(chuàng)新獎，是一家致力于樣品制備并獲得ISO 9001（產(chǎn)品質(zhì)量）、ISO 13485（產(chǎn)品可用于商業(yè)化的醫(yī)療設(shè)備，包括用于體外診斷領(lǐng)域）和ISO 15189（可用于臨床檢測機分子診斷領(lǐng)域的研發(fā)能力）認(rèn)證的生物科技公司。 目前該公司的產(chǎn)品多達(dá)700余種，提供一流的樣品制備試劑盒，應(yīng)用于基因組和蛋白組等不同的領(lǐng)域。其產(chǎn)品主要覆蓋包括各種標(biāo)本類型的DNA，RNA和蛋白質(zhì)的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。