中文名稱

血漿/血清RNA純化大提試劑盒（提取試劑盒）

英文名字

Plasma/Serum RNA Purification Maxi Kit

供應商

Norgen Biotek

產品貨號

NGB-56200

背景資料

Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

產品描述

This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here.The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

產品特點

Isolate all sizes of circulating and exosomal RNA, including microRNA; Versatile plasma/serum input ranging from 2 mL to 5 mL; No phenol extractions; No carrier RNA; Bind and elute all RNA irrespective of size or GC content, without bias; Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 50 μL to 100 μL; Purify high-quality RNA in 35-40 minutes; Formerly Cat. 30000.

保存建議

溶液密封室溫(15-25°C)保存，未開封至少保存2年。具體保存情況收到產品后請按說明書存放。

其他

Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術公司，基于專利的碳化硅膜純化技術和無苯酚專利配方，生產高品質的核酸和蛋白質純化及富集產品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發(fā)的科技創(chuàng)新獎，是一家致力于樣品制備并獲得ISO 9001（產品質量）、ISO 13485（產品可用于商業(yè)化的醫(yī)療設備，包括用于體外診斷領域）和ISO 15189（可用于臨床檢測機分子診斷領域的研發(fā)能力）認證的生物科技公司。 目前該公司的產品多達700余種，提供一流的樣品制備試劑盒，應用于基因組和蛋白組等不同的領域。其產品主要覆蓋包括各種標本類型的DNA，RNA和蛋白質的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。