背景資料

Mouse IgG TrueBlot? ULTRA is a unique Anti-Mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot? ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blot data with Mouse IgG TrueBlot? ULTRA, simply substitute the conventional Anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot? ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot? ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Mouse IgG TrueBlot? ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot? ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

應用類型

IF Microscopy,Immunoprecipitation,Western Blot,

免疫原

該Mouse TrueBlot? ULTRA: Anti-Mouse Ig HRP - 18-8817-30的詳細信息查看Rockland提供的產(chǎn)品說明書。

來源宿主

Rat

反應性

Mus musculus (Mouse)

保存建議

Store at -20 °C. This product is guaranteed for 1 year upon receipt, when handled and stored as instructed.

其他

Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國費城郊區(qū)的農(nóng)場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。