Anti Active Rac1-GTP Mouse Monoclonal Antibody

價格	￥4800
包裝	1管

最小起訂量	1管
發(fā)貨地	湖北
更新日期	2022-04-18

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產(chǎn)品詳情

中文名稱:Anti Active Rac1-GTP Mouse Monoclonal Antibody	英文名稱:Anti Active Rac1-GTP Mouse Monoclonal Antibody
保存條件: -20℃	純度規(guī)格: 1 mg/ml
產(chǎn)品類別: 單克隆抗體活性單抗
Applications:: IP, IHC

Active Rac1 GTP [26903] ￥：4800.00

[30 μL]

Catalog Number: 26903

Gene Symbol: RAC1

Description: Anti Active Rac1-GTP Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Immunogen: Recombinant full length protein of active Rac1

Applications: IP, IHC

Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgM

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti Rac of the cell lysates used (5% of that used in the top panel).

Immunofluorescence:

Immunofluorescence for the active Rac using Anti Active Rac1 GTP Mouse Monoclonal Antibody [26903] shows Rac GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections.
The tissue sections were fixed with -20 oC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti mouse AlexaFluor488 and anti rabbit AlexaFluor594 at 1:1000.

關(guān)鍵字： Rac;Rac1;Rac1-GTP;Active Rac1-GTP;Rac1-GTP antibody;

公司簡介

武漢戴安生物技術(shù)有限公司座落于武漢光谷生物城生物技術(shù)研究院，是一家由資深免疫學(xué)專家和海外留學(xué)人員創(chuàng)建的高科技生物技術(shù)企業(yè)。公司專注于體外診斷（IVD）試劑及其核心原料的研發(fā)生產(chǎn)及技術(shù)服務(wù)工作, 已成功建立了從IVD核心原料到試劑生產(chǎn)的研發(fā)生產(chǎn)路線，完成了原料開發(fā)-診斷試劑研發(fā)-診斷試劑放大生產(chǎn)的整個技術(shù)流程構(gòu)建，擁有獨(dú)立自主的抗原抗體的研發(fā)、放大生產(chǎn)技術(shù)平臺及生化試劑大包裝研發(fā)生產(chǎn)平臺。　　公司擁有完善的蛋白質(zhì)表達(dá)體系，抗體制備體系，研發(fā)人員擁有十余年從業(yè)經(jīng)驗，技術(shù)積累深厚，設(shè)備精良完善，在重組蛋白質(zhì)表達(dá)純化、天然蛋白質(zhì)提取、抗體研發(fā)和生產(chǎn)方面達(dá)到行業(yè)頂尖水平，也為客戶提供專業(yè)的抗體定制及相關(guān)技術(shù)服務(wù)，包括科研用多克隆抗體和單克隆抗體定制，藥物阻斷用抗體篩選，中和抗體制備等技術(shù)服務(wù)。我們秉承“精益求精，至善至美”的使命，追求“便捷，精確，真實”的客戶體驗，以創(chuàng)新推動產(chǎn)業(yè)發(fā)展，以實干滿足市場需求。

成立日期	2019-01-29 （6年）	注冊資本	100萬
員工人數(shù)	1-10人	年營業(yè)額	￥ 300萬-500萬
主營行業(yè)	生物化工,生化試劑,抗體,蛋白組學(xué),診斷試劑	經(jīng)營模式	貿(mào)易,工廠,試劑,定制,服務(wù)

武漢天德生物科技有限公司

非會員

公司成立：6年
注冊資本：100萬
企業(yè)類型：有限公司
主營產(chǎn)品：cAMP ELISA kit,cGMP kit,活性抗體,點(diǎn)突變抗體,組織芯片,多肽合成,rac1-GTP,Rhoa-GTP,cdc42-GTP,IDH1(R132H),ras(G12D)
公司地址：武漢市高新大道666號光谷生物城創(chuàng)新園武漢生物技術(shù)研究院B8棟

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