肌球蛋白重鏈11(MYH11)重組蛋白
[ PROPERTIES ]
Residues: Ala162~Gln315 linked by VPL
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q66K75
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5
Predicted Molecular Mass: 19.2kDa
Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducingconditions.Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VP L AYKGREEAI KQ L RK LQAQM KDFQRE LE DA RAS R DEI FAT AKE NEKKAKSLEADLMQLQE DLAAAERARK QADLEKEELA EELASSLSGR NALQDEKRRL EARIAQLEGELEEEQGNMEA MSDRVRKATQ QAEQLSNELA TERSTAQKNE SARQQ
特別提示:本公司的所有產(chǎn)品僅可用于科研實(shí)驗(yàn),嚴(yán)禁用于臨床醫(yī)療及其他非科研用途!
關(guān)鍵字: AAT4;FAA4;SMHC;肌球蛋白重鏈11(MYH11)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日,注冊(cè)地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號(hào)。
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