尿嘧啶磷酸核糖轉(zhuǎn)移酶(UPRT)重組蛋白
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Asn75~Ala297 (Accession # Q96BW1),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Nucleus.
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.2
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 51.8kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GS T S G S G H H H HH HSA G LVP R GS TA IG MK E T AA AK F E R Q HM DSP D L G T L EV
LFQGPLGSEF-NSEGNS GSGDSSSYDA PAGNSFLEDC ELSRQIGAQL KLLPMNDQIR
ELQTIIRDKT ASRGDFMFSA DRLIRLVVEE GLNQLPYKEC MVTTPTGYKY EGVKFEKGNC
GVSIMRSGEA MEQGLRDCCR SIRIGKILIQ SDEETQRAKV YYAKFPPDIY RRKVLLMYPI
LSTGNTVIEA VKVLIEHGVQ PSVIILLSLF STPHGAKSII QEFPEITILT TEVHPVA
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關(guān)鍵字： FUR1;Uracil ;UPRT;尿嘧啶磷酸核糖轉(zhuǎn)移酶(UPRT)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競爭力的價格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實驗技術(shù)服務(wù)等多個應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實驗試劑，染色液等科研產(chǎn)品。