上皮細(xì)胞轉(zhuǎn)化序列2(ECT2)重組蛋白
[ PROPERTIES ]
Residues: Leu166~Arg348 (Accession # Q9H8V3),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Nucleus. Cytoplasm.
cytoskeleton, spindle. Cleavage furrow. Midbody.
Cell junction, tight junction. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and preservative.
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 22.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.
MGHHHHHHSGSEF-LVHHM GGVIRKDFNS KVTHLVANCT QGEKFRVAVS LGTPIMKPEWIYKAWERRNE QDFYAAVDDF RNEFKVPPFQ DCILSFLGFS DEEKTNMEEMTEMQGGKYLPLGDERCTHLV VEENIVKDLP FEPSKKLYVV KQEWFWGSIQ MDARAGETMY LYEKANTPELKKSVSMLSLN TPNSNRKR
[ REFERENCES ]
1.Tatsumoto T., et al. (1999) J. Cell Biol. 147:921-928.
2. Saito S., et al. (2003) J. Cell. Biochem. 90:819-836.
3. Saito S., et al. (2004) J. Biol. Chem. 279:7169-7179.
4. Liu X.F., et al. (2004) Mol. Cell. Biol. 24:6665-6675.
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關(guān)鍵字： Cell;Sequence;ECT2;上皮細(xì)胞轉(zhuǎn)化序列2(ECT2)重組蛋白;
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