肉毒堿乙酰轉移酶(CRAT)重組蛋白
[ PROPERTIES ]
Residues: Lys363~Leu626 (Accession # P43155),
with two N-terminal Tags, His-tag and T7-tag.
Host: E. coli
Subcellular Location: Endoplasmic reticulum.
Peroxisome. Mitochondrion inner membrane;
Peripheral membrane protein; Matrix side. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.6
Predicted Molecular Mass: 33.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KKPELVRS PLVPLPMPKK
LRFNITPEIK SDIEKAKQNL SIMIQDLDIT VMVFHHFGKD FPKSEKLSPD AFIQMALQLA
YYRIYGQACA TYESASLRMF HLGRTDTIRS ASMDSLTFVK AMDDSSVTEH QKVELLRKAV
QAHRGYTDRA IRGEAFDRHL LGLKLQAIED LVSMPDIFMD TSYAIAMHFH LSTSQVPAKT
DCVMFFGPVV PDGYGVCYNP MEAHINFSLS AYNSCAETNA ARLAHYLEKA LLDMRALLQS
HPRAKL
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關鍵字： CAT1;Carnitine;acetylase;肉毒堿乙酰轉移酶(CRAT)重組蛋白;
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