周期素依賴性激酶抑制因子3(CDKN3)重組蛋白
[ PROPERTIES ]
Residues: Met1~Arg212
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q16667
Host: E. coli
Subcellular Location: Cytoplasm, perinuclear
region.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 53.8kDa
Accurate Molecular Mass: 58kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MKPPSSIQTS EFDSSDEEPI EDEQTPIHIS WLSLSRVNCS QFLGLCALPG CKFKDVRRNV
QKDTEELKSC GIQDIFVFCT RGELSKYRVP NLLDLYQQCG IITHHHPIAD GGTPDIASCC
EIMEELTTCL KNYRKTLIHC YGGLGRSCLV AACLLLYLSD TISPEQAIDS LRDLRGSGAI
QTIKQYNYLH EFRDKLAAHL SSRDSQSRSV SR
特別提示：本公司的所有產(chǎn)品僅可用于科研實驗，嚴禁用于臨床醫(yī)療及其他非科研用途！
關鍵字： CDI1;CIP2;KAP1;周期素依賴性激酶抑制因子3(CDKN3);
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
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