中文名稱

RbcL | Rubisco陽性對(duì)照/定量標(biāo)準(zhǔn)

英文名字

RbcL|Rubisco positive control/quantitation standard

供應(yīng)商

Agrisera

產(chǎn)品貨號(hào)

AS01-017S

產(chǎn)品報(bào)價(jià)

￥3141.6/100ul

產(chǎn)品說明書

點(diǎn)擊查看

購買方式

銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868。

產(chǎn)品新聞

背景資料

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.Source of Rubisco standard: Rubisco protein was purified directly from a plant tissue - spinach.

產(chǎn)品描述

Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/ulProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA? Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.Please, use the 55 kDa size of RbcL for calculations. The pmoles in the standard refer to pmoles of rbcL monomers.

產(chǎn)品特點(diǎn)

保存建議

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

其他

Agrisera公司提供1000種用于植物和藻類細(xì)胞生物學(xué)研究的一抗，2000多種二抗。Agrisera公司提供的植物領(lǐng)域的抗體涵蓋擬南芥、萊茵衣藻、藍(lán)藻、松柏類、硅藻、大豆、大麥、蒺藜苜蓿、煙草、稻、菜豆、小立碗蘚、楊樹、豌豆、番茄、馬鈴薯、菠菜、小麥、玉米、葡萄等多個(gè)物種，此外還有水生生物類（藻類、魚）抗體，細(xì)菌、昆蟲、真菌抗體。針對(duì)植物蛋白質(zhì)的保守氨基酸序列，Agirsera還獨(dú)家開發(fā)了數(shù)十種通用抗體（global antibodies），這類抗體幾乎可以與所有的高等植物及藻類產(chǎn)生免疫反應(yīng)，可為研究人員提供極為方便的選擇。