脂肪酸結(jié)合蛋白5(FABP5)重組蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Fatty Acid Binding Protein 5, Epidermal
Gene Names: FABP5
Size: 100μg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: High specificity for fatty acids. Highest affinity for C18 chain length.
Decreasing the chain length or introducing double bonds reduces the affinity. May
be involved in keratinocyte differentiation.
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm.
Tissue Specificity: Specificity: Specificity: Specificity: Keratinocytes; highly expressed in psoriatic skin.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Ala2~Glu135 (Accession # Q01469), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 16.3 kDa as determined by SDS-PAGE reducing
conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 16.3 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGS-ATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL
KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCTRI
YEKVE
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Madsen P.S., et al. (1992) J. Invest. Dermatol. 99:299-305.
2. Gutierrez-Gonzalez L.H., et al. (2002) Biochem. J. 364:725-737.
3. Hohoff C., et al. (1999) Biochemistry 38:12229-12239.
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關(guān)鍵字： E-FABP;EFABP;PA-FABP;脂肪酸結(jié)合蛋白5(FABP5)重組蛋白;
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上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競(jìng)爭(zhēng)力的價(jià)格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實(shí)驗(yàn)技術(shù)服務(wù)等多個(gè)應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測(cè)試劑盒、PCR檢測(cè)試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實(shí)驗(yàn)試劑，染色液等科研產(chǎn)品。