前列腺素I合酶(PTGIS)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Met1~Pro500
Tags: N-terminal His-Tag
Tissue Specificity: Ovary, Heart, Lung. Subcellular Location: Endoplasmic reticulum membrane. Single-pass
membrane protein. Purity: >95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.8
Predicted Molecular Mass: 60.8kDa
Accurate Molecular Mass: 61kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed. The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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關(guān)鍵字： PGIS;CYP8A1;Prostacyclin;前列腺素I合酶(PTGIS)重組蛋白;
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