中文名稱

Cdc42 Pulldown 活化檢測試劑盒

英文名字

Cdc42 Pulldown Activation Assay Kit

供應(yīng)商

Cytoskeleton

產(chǎn)品貨號

BK034-S

產(chǎn)品報價

￥5611.84/KIT20assays

產(chǎn)品說明書

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購買方式

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產(chǎn)品新聞

背景資料

該Cdc42 Pulldown 活化檢測試劑盒產(chǎn)品是Cytoskeleton品牌原裝進口，Cytoskeleton品牌是細(xì)胞骨架研究專家，專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。艾美捷作為中國區(qū)域的總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨立專業(yè)技術(shù)支持團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù)，在意您的成功！

產(chǎn)品描述

收到該Cdc42 Pulldown 活化檢測試劑盒Cdc42 Pulldown Activation Assay Kit產(chǎn)品后，查看廠家提供的產(chǎn)品說明書詳細(xì)介紹。

產(chǎn)品特點

The Rho switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of the GTPases is of obvious biological significance and is a subject of intense investigation. The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Rac and Cdc42 protein activation. The assay uses the Cdc42/Rac Interactive Binding (CRIB) region (also called the p21 Binding Domain, PBD) of the Cdc42 / Rac effector protein, p21 activated kinase 1 (PAK). The CRIB/PBD protein motif has been shown to bind specifically to the GTP-bound form of Rac and/or Cdc42 proteins. The fact that the PBD region of PAK has a high affinity for both GTP-Rac and GTP-Cdc42 and that PAK binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of both Rac and Cdc42 make it an ideal tool for affinity purification of GTP-Rac and GTP-Cdc42 from cell lysates. The PAK-PBD protein supplied in this kit corresponds to residues 67-150. This includes the highly conserved CRIB region (aa 74-88) plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42. The PAK-PBD is in the form of a GST fusion protein, which allows one to "pull-down" the PAK-PBD/GTP-Cdc42 (or GTP-Rac) complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Rac/Cdc42 activation in cells. The amount of activated Cdc42 is determined by a Western blot using a Cdc42 specific antibody.

保存建議

廠家推薦常溫運輸，收到產(chǎn)品后建議保存于4°C。

其他

Cytoskeleton公司成立于1993年，專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。公司提供藥物篩選、信號轉(zhuǎn)導(dǎo)、蛋白質(zhì)轉(zhuǎn)錄后修飾（PTM）、細(xì)胞骨架研究相關(guān)的系列試劑盒和產(chǎn)品，尤其以細(xì)胞骨架相關(guān)研究見長，既能滿足于樣品較少的科學(xué)研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動蛋白，小G蛋白，GAPs，GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務(wù)。