粒細胞巨噬細胞集落刺激因子(GM-CSF)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ala18~Glu144
Tags: Two N-terminal Tags, His-tag and GST-tag
Tissue Specificity: Blood. Subcellular Location: Secreted. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 41.5kDa
Accurate Molecular Mass: 45kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as
follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
關(guān)鍵字： CSF2;GMCSF;Sargramostim;粒細胞巨噬細胞集落刺激因子(GM-CSF;
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