絲聚蛋白2(FLG2)重組蛋白
[ PROPERTIES ]
Residues: Val1915~Arg2143
Tags: N-terminal His-Tag
Accession: Q5D862
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.7
Predicted Molecular Mass: 25.4kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VHKRHQ TTHGQTGDTT EHGHPSHGQT IQTGSRTTGR RGSGHSEYSD SEGPSGVSHT
HSGHTHGQAG SHYPESGSSV HERHGTT HGQ TA DTTRHGHS GHGQSTQRGS
RTTGRRASGH SEYS DSEGHS GVSHTHSGHA HGQAGSQHGE SGSSVHERHG
TT HGQTGDT T RHAHSGHG QS TQRGSRTAGR RGSG HSESS D SEV HSGVS HT
HSGHTYGQAR SQHGESGSAI HGR
[ REFERENCES ]
1. Bian Y., et al. (2014) J. Proteomics 96:253-262.
2. Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17.
3. Wu Z. (2004) Submitted to the EMBL/GenBank/DDBJ databases.
4. Listwan P., Rothnagel J.A. (2005) Submitted to the EMBL/GenBank/DDBJ
databases.
關(guān)鍵字： IFPS;Ifapsoriasin;Intermediate;絲聚蛋白2(FLG2)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競爭力的價(jià)格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實(shí)驗(yàn)技術(shù)服務(wù)等多個應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實(shí)驗(yàn)試劑，染色液等科研產(chǎn)品。