MCC950 Sodium

別名: CP-456773 Sodium, CRID3 Sodium

目錄號：S7809 Purity: 99.98%

MCC950 Sodium是一種有效的、選擇性NLRP3抑制劑，在骨髓來源的巨噬細(xì)胞中IC50為7.5 nM。對AIM2, NLRC4或NLRP1炎癥體沒有作用。

MCC950 Sodium Chemical Structure

CAS: 256373-96-3

規(guī)格	價格	庫存
10mg	877.22	現(xiàn)貨
50mg	3661.44	現(xiàn)貨
200mg	8165.69	現(xiàn)貨
1g	12858.3	現(xiàn)貨
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產(chǎn)品質(zhì)控

批次：純度： 99.98%

99.98

MCC950 Sodium相關(guān)產(chǎn)品

相關(guān)產(chǎn)品	MCC950 MCC950 CY-09 INF39 NLRP3 Inflammasome Inhibitor I Dapansutrile	點擊展開
相關(guān)化合物庫	激酶抑制劑庫 FDA藥物庫天然產(chǎn)物庫已知活性藥物庫-I 高選擇性抑制劑庫	點擊展開

細(xì)胞實驗數(shù)據(jù)示例

細(xì)胞系	實驗類型	給藥濃度	孵育時間	活性描述	文獻(xiàn)信息
16HBE cells	Cell viability assay	0.01, 0.1, 1, 10, 100 μM	24, 48, 72h	100μM MCC950 functionally alleviated silica-induced activation of NLRP3 inflammasome in 16 HBE cells.	29258746
INS-1 cells	Function assay	0.01 μM	1 h	MCC950 inhibited not only inflammasome activation but also Ang II-induced IL-1β elevation and cell apoptosis in INS-1 cells.	30939192
THP-1 monocytes	Function assay	1 μM	30 mins	Pretreatment of THP‐1 monocytes for 30 min with this compound resulted in a significant reduction in IL‐1β release, but not pyroptosis upon transfection with LPS.	26173988
mouse BMDM cells	Function assay		30 mins	Inhibition of NLRP3 inflammasome activation in LPS-stimulated mouse BMDM assessed as reduction in NLRP3 inflammasome-induced IL-1beta production preincubated for 30 mins followed by NLRP3 stimulation with ATP by ELISA. IC50=0.0075 μM	27994733
mouse BMDM cells	Function assay		30 mins	Inhibition of NLRP3 in mouse BMDM cells preincubated for 30 mins followed by ATP addition by ELISA analysis. IC50=0.0075 μM	26422006
macrophages	Function assay		30 mins	Inhibition of NLRP3 in human monocyte derived macrophages assessed as reduction in LPS-induced IL-1beta production incubated for 30 mins followed by nigericin sodium salt stimulation for 2 hrs post LPS challenge for 3 hrs by ELISA, IC50 = 0.005 μM.	29338910
macrophages	Function assay			Inhibition of NLRP3 in human monocyte derived macrophages assessed as reduction in LPS-induced IL-1beta production by ELISA, IC50 = 0.008 μM.	29338910
點擊查看更多細(xì)胞系數(shù)據(jù)

生物活性

產(chǎn)品描述

MCC950 Sodium是一種有效的、選擇性NLRP3抑制劑，在骨髓來源的巨噬細(xì)胞中IC50為7.5 nM。對AIM2, NLRC4或NLRP1炎癥體沒有作用。

靶點

NLRP3 ^[1] (in BMDM (bone marrow derived macrophages))	NLRP3 ^[1] (in HMDM)
7.5nM	8.1 nM

體外研究(In Vitro)
體外研究活性	MCC950在骨髓來源的巨噬細(xì)胞和人類單核細(xì)胞衍生的巨噬細(xì)胞中，抑制經(jīng)典和非經(jīng)典NLRP3激活，IC50分別為7.5和8.1 nM。MCC950特異性抑制NLRP3的激活，不抑制AIM2, NLRC4或NLRP1這些炎癥小體^[1]。
實驗圖片	檢測方法	檢測指標(biāo)	實驗圖片	PMID
	Western blot	NLRP3 / Casp-1 / BMI1 / ALDH1 / CD44		28865486
	Growth inhibition assay	Cell viability		27670879
	Immunofluorescence	ALDH1 / BIM1 / CD44		28865486
	ELISA	IL-1β		28726778

體內(nèi)研究(In Vivo)
體內(nèi)研究活性	MCC950減少白介素-1β(IL-1β)的產(chǎn)生、減輕實驗性自身免疫性腦脊髓炎的嚴(yán)重性。在自身炎癥性疾病Cryopyrin蛋白相關(guān)周期性綜合征的小鼠模型中，MCC950能夠挽救其新生時期的死亡。在間接體外實驗中，在來源于韋二氏綜合征患者的樣品具有活性^[1]。
動物實驗	Animal Models	C57BL/6小鼠
	Dosages	50 mg/kg
	Administration	腹腔注射

參考文獻(xiàn)
[1] Coll RC, et al. Nat Med. 2015, 21(3):248-55. [2] Meng L, et al. Cell Death Dis.?2022 Jan 10;13(1):38.

參考文獻(xiàn)

[1] Coll RC, et al. Nat Med. 2015, 21(3):248-55.
[2] Meng L, et al. Cell Death Dis.?2022 Jan 10;13(1):38.

化學(xué)信息&溶解度

分子量	426.46	分子式	C₂₀H₂₃N₂O₅S.Na
CAS號	256373-96-3	SDF	Download MCC950 Sodium SDF
Smiles	CC(C)(C1=COC(=C1)S(=O)(=O)[N-]C(=O)NC2=C3CCCC3=CC4=C2CCC4)O.[Na+]
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復(fù)凍融！	1年-80°C溶于溶劑
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復(fù)凍融！	1個月-20°C溶于溶劑

體外溶解度
批次：

Water : 43 mg/mL (100.83 mM)

Ethanol : 43 mg/mL (100.83 mM)