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SAG (Smoothened Agonist) HCl

SAG (Smoothened Agonist) HCl是一種細(xì)胞滲透性Smoothened (Smo)激動(dòng)劑,其在Shh-LIGHT2細(xì)胞中的EC50約為3 nM。

SAG (Smoothened Agonist) HCl Chemical Structure

SAG (Smoothened Agonist) HCl Chemical Structure

CAS: 2095432-58-7

規(guī)格 價(jià)格 庫存 購買數(shù)量
10mM (1mL in DMSO) 2432.43 現(xiàn)貨
2mg 954.43 現(xiàn)貨
5mg 2024.65 現(xiàn)貨
25mg 6110.42 現(xiàn)貨
100mg 7944.3 現(xiàn)貨
1g 57248.1 現(xiàn)貨
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SAG (Smoothened Agonist) HCl相關(guān)產(chǎn)品

相關(guān)信號通路圖

細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例

細(xì)胞系 實(shí)驗(yàn)類型 給藥濃度 孵育時(shí)間 活性描述 文獻(xiàn)信息
HUVEC Cytotoxicity assay 24 h Cytotoxicity against HUVEC after 24 hrs by WST-1 assay, IC50=22 μM 22985958
mouse Shh-Light II cell Function assay 48 hrs Activation of Shh pathway in mouse Shh-Light II cells assessed as induction of gli1 expression after 48 hrs by renilla luminescence assay, EC50=0.0088 μM 22985958
Shh-Light II Function assay 48 hrs Activation of Shh pathway in mouse Shh-Light II cells assessed as induction of gli1 expression after 48 hrs by renilla luminescence assay, EC50 = 0.0618 μM. 22985958
C3H10T1/2 Function assay 6 days Agonist activity at Smo in mouse C3H10T1/2 cells assessed as induction of cell differentiation into osteoblast incubated for 6 days by alkaline phosphatase assay, EC50 = 0.13 μM. 27429255
NIH/3T3 Function assay Agonist activity at Smo in mouse NIH/3T3 cells transfected with Gli-dependent firefly luciferase reporter assessed as stimulation of Gli transcription factor, EC50 = 0.03 μM. 27429255
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
RD qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for RD cells 29435139
SK-N-SH qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells 29435139
MG 63 (6-TG R) qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells 29435139
NB1643 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells 29435139
Rh18 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh18 cells 29435139
點(diǎn)擊查看更多細(xì)胞系數(shù)據(jù)

生物活性

產(chǎn)品描述 SAG (Smoothened Agonist) HCl是一種細(xì)胞滲透性Smoothened (Smo)激動(dòng)劑,其在Shh-LIGHT2細(xì)胞中的EC50約為3 nM。
靶點(diǎn)
Smoothened [1]
(mouse cultured cell assay)
3 nM(EC50)
體外研究(In Vitro)
體外研究活性

SAG通過直接結(jié)合到Smo螺旋束調(diào)控Smo活性。[1]

SAG以GRK2依賴的方式誘導(dǎo)通過Gli的Smo-依賴性信號。[2]

SAG(1 nM)也會誘導(dǎo)神經(jīng)元和神經(jīng)膠質(zhì)前體細(xì)胞的增殖,而不影響新生細(xì)胞的分化模式。[3]

實(shí)驗(yàn)圖片 檢測方法 檢測指標(biāo) 實(shí)驗(yàn)圖片 PMID
Western blot LC3I / LC3II 23504944
Immunofluorescence Smo acetylated alpha tubulin 25636740
體內(nèi)研究(In Vivo)
體內(nèi)研究活性

在成年大鼠海馬中,SAG (2.5 nM)腦室內(nèi)給藥顯著增加新生細(xì)胞的數(shù)量,并延長海馬細(xì)胞的存活時(shí)間。[3]

在小鼠中,SAG (20 μg/g, i.p.)有效防止GC-誘導(dǎo)的新生小腦發(fā)育異常。[4]

動(dòng)物實(shí)驗(yàn) Animal Models 大鼠
Dosages 2.5 nM
Administration 側(cè)腦室給藥
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05937178 Recruiting
Hepatitis B Chronic
Huashan Hospital|The First Affiliated Hospital of Anhui Medical University|Anhui Provincial Hospital|Beijing YouAn Hospital|Peking University People''s Hospital|Peking University First Hospital|Xiamen Hospital of Traditional Chinese Medicine|First Affiliated Hospital of Fujian Medical University|LanZhou University|Third Affiliated Hospital Sun Yat-Sen University|First Affiliated Hospital of Guangxi Medical University|The Affiliated Hospital Of Guizhou Medical University|Hainan General Hospital|Hebei Medical University Third Hospital|The First Affiliated Hospital of Henan University of Traditional Chinese Medicine|Henan Provincial People''s Hospital|The Second Affiliated Hospital of Harbin Medical University|Tongji Hospital|Wuhan Union Hospital China|Renmin Hospital of Wuhan University|Central South University|Xiangya Hospital of Central South University|The First Hospital of Jilin University|the Second Hospital of Nangjing|The Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School|The First Affiliated Hospital with Nanjing Medical University|The Affiliated Hospital of Xuzhou Medical University|The First Affiliated Hospital of Nanchang University|Shengjing Hospital|Qingdao Sixth People''s Hospital|Shandong Provincial Hospital|The Second Hospital of Shandong University|The First Affiliated Hospital of Shanxi Medical University|Tang-Du Hospital|First Affiliated Hospital Xi''an Jiaotong University|Ruijin Hospital|West China Hospital|Tianjin Second People''s Hospital|First Affiliated Hospital of Xinjiang Medical University|The First People''s Hospital of Yunnan|Shulan (Hangzhou) Hospital|Zhejiang University|Southwest Hospital China|The Second Affiliated Hospital of Chongqing Medical University|Sichuan Provincial People''s Hospital
January 31 2023 --
NCT05701592 Recruiting
Posterior Cruciate Ligament Tear
Konrad Malinowski MD|Artromedical Konrad Malinowski Clinic
December 20 2022 --
NCT03645044 Active not recruiting
Hiv|HBV Coinfection
University of Melbourne|National Health and Medical Research Council Australia|The University of Western Australia|University of Adelaide|The HIV Netherlands Australia Thailand Research Collaboration|University of Malaya|YR Gaitonde Centre for AIDS Research and Education|Melbourne Health
May 24 2018 --

化學(xué)信息&溶解度

分子量 526.52 分子式

C28H29Cl2N3OS

CAS號 2095432-58-7 SDF Download SAG (Smoothened Agonist) HCl SDF
Smiles CNC1CCC(CC1)N(CC2=CC(=CC=C2)C3=CC=NC=C3)C(=O)C4=C(C5=CC=CC=C5S4)Cl.Cl
儲存條件(自收到貨起)

體外溶解度
批次:

DMSO : 82 mg/mL ( (155.73 mM) ;DMSO吸濕會降低化合物溶解度,請使用新開封DMSO)

Water : 50 mg/mL (94.96 mM)

Ethanol : 27 mg/mL (51.28 mM)

摩爾濃度計(jì)算器

體內(nèi)溶解度
批次:

現(xiàn)配現(xiàn)用,請按從左到右的順序依次添加,澄清后再加入下一溶劑

動(dòng)物體內(nèi)配方計(jì)算器

實(shí)驗(yàn)計(jì)算

摩爾濃度計(jì)算器

質(zhì)量 濃度 體積 分子量

動(dòng)物體內(nèi)配方計(jì)算器(澄清溶液)

第一步:請輸入基本實(shí)驗(yàn)信息(考慮到實(shí)驗(yàn)過程中的損耗,建議多配一只動(dòng)物的藥量)

mg/kg g μL

第二步:請輸入動(dòng)物體內(nèi)配方組成(配方適用于不溶于水的藥物;不同批次藥物配方比例不同,請聯(lián)系Selleck為您提供正確的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計(jì)算結(jié)果:

工作液濃度: mg/ml;

DMSO母液配制方法: mg 藥物溶于μL DMSO溶液(母液濃度mg/mL,:如該濃度超過該批次藥物DMSO溶解度,請先聯(lián)系Selleck);

體內(nèi)配方配制方法:μL DMSO母液,加入μL PEG300,混勻澄清后加入μL Tween 80,混勻澄清后加入μL ddH2O,混勻澄清。

體內(nèi)配方配制方法:μL DMSO母液,加入μL Corn oil,混勻澄清。

注意:1. 首先保證母液是澄清的;
2.一定要按照順序依次將溶劑加入,進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液,可采用渦旋、超聲或水浴加熱等物理方法助溶。

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