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Brefeldin A (BFA)

別名: Cyanein, Decumbin 中文名稱：布雷菲德菌素A

Brefeldin A (BFA)作用于HCT 116細(xì)胞，抑制內(nèi)酯抗生素和ATPase，作用于protein transport（蛋白轉(zhuǎn)運(yùn)），IC50為0.2 μM，誘導(dǎo)癌細(xì)胞分化和凋亡。它還能提高同源重組修復(fù)效率，是CRISPR-mediated HDR的增強(qiáng)劑。Brefeldin A 也是自噬和線粒體自噬的抑制劑。

Brefeldin A (BFA) Chemical Structure

Brefeldin A (BFA) Chemical Structure

CAS: 20350-15-6

規(guī)格 價(jià)格 庫存 購買數(shù)量
10mM (1mL in DMSO) 655.27 現(xiàn)貨
5mg 571.63 現(xiàn)貨
25mg 2235.02 現(xiàn)貨
100mg 5490.64 現(xiàn)貨
1g 28665 現(xiàn)貨
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Brefeldin A (BFA)相關(guān)產(chǎn)品

相關(guān)信號(hào)通路圖

ATPase Signaling Pathways

ATPase信號(hào)通路圖

細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例

細(xì)胞系 實(shí)驗(yàn)類型 給藥濃度 孵育時(shí)間 活性描述 文獻(xiàn)信息
HeLa? Function Assay 5 μg/ml 3 h causes nuclear exclusion of the FoxO1 transcription factor and decreases transcription of FoxO1-regulated genes 24843827
3T3-L1 Function Assay 5 μg/ml 1?h causes phosphorylation of the FoxO1 transcription factor 24843827
3T3-L1 Function Assay 5 μg/ml 1?h causes redistribution of GLUT4 but not increase in glucose uptake 24843827
3T3-L1 Function Assay 5 μg/ml 30 min causes reversible redistribution of GLUT4 24843827
3T3-L1 Function Assay 5 μg/ml 30 min recapitulates insulin action with respect to regulating Akt activity and AS160 phosphorylation 24843827
3T3-L1 Function Assay 5 μg/ml 30 min mimics the effects of insulin and causes robust phosphorylation of Akt (Ser 473) and phosphorylation of AS160 (Thr 642 and Ser 588) 24843827
H838-KDLKB1? Function Assay 30?ng/ml 12/18 h increases the levels of phosphorylated eIF2α (phospho-eIF2α) 25011082
H838-KDLKB1? Function Assay 30?ng/ml 12/18 h increases the protein levels of BiP? 25011082
H838-LKB1 Function Assay 30?ng/ml 12/18 h increases the protein levels of BiP? 25011082
HepG2? Function Assay 1μg/mL 3–24?h increases the level of APE1 in a time-dependent manner 25026174
Huh-7? Function Assay 1μg/mL 3–24?h increases the level of APE1 in a time-dependent manner 25026174
RBE4 Function Assay 2?μM 3–24?h induces an overload of Ca2+?in the mitochondria in the first 6?h of incubation (p?<?0.001) but Ca2+?levels in this organelle decreased after 12?h of incubation 25128025
RBE4 Function Assay 2?μM 3–24?h induces a delayed depletion of the ER Ca2+?content at 6?h of incubation significantly 25128025
RBE4 Function Assay 2?μM 3–24?h increases the levels of ROS time-dependently 25128025
RBE4 Function Assay 2?μM 3–24?h increases active caspase-12 in a time-dependent manner? 25128025
RBE4 Function Assay 2?μM 3–24?h increases the XBP1 protein levels after 3 and 6?h of treatment 25128025
RBE4 Apoptosis Assay 2?μM 3–24?h induces apoptosis time dependently 25128025
HEK293/hERG Function Assay 10?μM 1?h results in a time-dependent reduction mature hERG protein? 25218469
MEC Function Assay 1 μM 1.5 h causes a dramatic decrease in the surface VEGFR2 25228815
KMS-6 Function Assay 1?μM? 24 h exhibits half the secretion of galanin-LI as did the control 25229126
A172 Function Assay 10?μg/ml 4?h results in the retrograde transport of fluorescent granules 25239507
MDA-MB-231 Function Assay 0–50 μg/mL 24 h inhibits the formation of 3D and 2D colonies 25356567
MDA-MB-231 Apoptosis Assay 0.05–1 μg/mL 24 h induces PARP (poly ADP-ribose polymerase-1) cleavage 25356567
MDA-MB-231 Growth Inhibition Assay 0.01/0.05 μg/mL 24 h increases the fraction of sub-G1 cell debris 25356567
MDA-MB-231 Apoptosis Assay 0.1 μg/mL 4 h induces apoptosis 25356567
MDA-MB-231 Cell Viability Assay 0–50 μg/mL 48 h EC50?= 0.016 μg/mL 25356567
H1299 Function Assay 10 μg/ml 24 h induces autophagy? 25388970
PEXEL-Nluc Function Assay 5 mg/mL 6 h causes an increase in reporter activity in the parasite 25392998
SP-Nluc Function Assay 5 mg/mL 6 h causes an increase in reporter activity in the parasite 25392998
iPSC-CMs? Function Assay 500 ng/ml 48 h increases the intensity of the higher mobility LAMPs at the cost of the lower mobility species 25488666
OB-6 Apoptosis Assay 2.7?μM 48 h induces apoptosis 25532480
SMCs Function Assay 1μg/mL 3 h accumulates CNPY2 protein in the ER compartment and no longer co-localized with the Golgi marker? 25589425
HepG2? Function Assay 1?μM? 24 h decreases the level of PXR mRNA 25616597
FRT? Function Assay 5 μg/ml 2?h prevents the increase in cleaved α subunits when [Na+]i?was reduced 25767115
FRT? Function Assay 5 μg/ml 2?h blocks trafficking through the Golgi complex by inhibiting ER-to-Golgi transport 25767115
nHDFs? Function Assay 1?μM? 2?h prevents the assembly of cytosolic coat proteins onto Golgi membranes 25772616
DF1? Function Assay 1?μM? 48 h disperses?the exogenous CSGalNAcT2 protein 25807054
COS Function Assay 1 μg/ml 3 h completely disperses the AP-1 signal? 25915900
HeLa Function Assay 200?ng/ml 3 h induces the artificial break-up of the Golgi complex 25948586
NRK Function Assay 200?ng/ml 4?h rescues mitotic progression 25948586
Caco-2 Function Assay 2.5 μM 30 min attenuates the TGF-β1-mediated increase in SERT function 25954931
HUVEC Function Assay 10?μM 1?h increases?the number and intensity of fluorescent areas especially in perinuclear space 25956988
HUVEC Function Assay 10?μM 1?h abolishes hypoxia-induced release of ATP from apical and basolateral surfaces 25956988
HEMC-1 Function Assay 0.1?μg/ml 24?h causes a higher inhibitory effect on exocytosis than nocodazole 25972759
SMCs Function Assay 10?μg/ml 0-12 h causes a transient Ca2+?release from the ER/SR? 26172080
SMCs Function Assay 10?μg/ml 0-12 h shows a trend towards a higher concentration of the ER/SR network in the perinuclear area? 26172080
MEFs VAMP7 KO Function Assay 5?μM 20 min causes resident enzymes such as NAGT-GFP, to diffuse back to the ER 26196023
MEFs WT Function Assay 5?μM 20 min causes resident enzymes such as NAGT-GFP, to diffuse back to the ER 26196023
C2C12 Function Assay 1?μg/ml 1?h abolishes cytokine release from C2C12 myotubes 26291279
PC12 Function Assay 2 μM 1 h inhibits the L-DOPA (20?μM)-induced transient ERK1/2 phosphorylation? 26363191
HEK293 Function Assay 5?μg/ml 12?h abolishes CMA-induced CRELD2 secretion 24687431
COS-1 Function Assay 5 μg/ml 24 h? restricts localization of NB in the perinuclear region? 24671751
RAW264.7 Apoptosis Assay 4 μM 48 h attenuates the inhibition of ox-LDL-induced apoptosis and the facilitation of cholesterol efflux by Ac-hE-18A-NH2 24639032
MDMs Apoptosis Assay 10?μg/ml 12/15 h induces apoptosis 24556695
PMHs? Function Assay 10–20?μg/ml 24?h induced ER stress 24407242
PMHs? Apoptosis Assay 10–20?μg/ml 24?h increases cell death 24407242
HEK293/tau Function Assay 5 μM 1/2/4 h induces Golgi fragmentation? 24368089
HEK293/tau Function Assay 5 μM 3 h induces tau hyperphosphorylation 24368089
ADF Function Assay 10 μM 16 h inhibits the ZnCl2-induced translocation of CRT 24228232
U373? Function Assay 10 μM 16 h inhibits the ZnCl2-induced translocation of CRT 24228232
RKO-HIPK2i Function Assay 10 μM 16 h inhibits the ZnCl2-induced translocation of CRT 24228232
ADF? Function Assay 10 μM? 6 h impairs the DC activation 24228232
Huh7 Function Assay 5 μg/ml 4 h abolishes the secretion of intracellular ApoB 24100140
Huh7 Function Assay 5 μg/ml 1 h causes a significant increase in ApoB-crescents 24100140
Huh7 Function Assay 5–10?ng/ml 12 h increases ApoB-crescents without inhibiting secretion 24100140
BAECs Function Assay 5 μg/ml 0-4 h induces the rapid dephosphorylation of eNOS at Ser1179 24085225
Macrophages Function Assay 71 μM 6 h inhibits lunasin internalization? 24039740
Colo 205 Growth Inhibition Assay 0-5 μg/mL 48 h inhibits cell growth in suspension cultures with an estimated IC50 of ~15 ng/mL 23973996
Colo 205 Function Assay 0.012-0.025 μg/mL 14 d reduces the clonogenicity of Colo 205 CSCs 23973996
Colo 205 Apoptosis Assay 0.1 μg/mL 0-24 h induces apoptosis of Colo 205 cells in suspension cultures 23973996
Colo 205 Function Assay 0.015 μg/mL 24 h induces the expression of ER stress-related genes 23973996
Colo 205 Function Assay 0.015 μg/mL 24 h inhibits the activity of MMPs 23973996
IBRS2 Function Assay 5 μg/ml 0.5 h disrupts the ERGIC and Golgi? 23963534
IBRS2 Function Assay 5 μg/ml 0.5 h enhances FMDV infection 23963534
HeLa Function Assay 2 μM 2 h? attenuates the TNF-induced secretion of IL-15 23950892
HFS? Function Assay 0-1 μg/ml 24 h GLTP expression reaches a plateau at concentrations as low as 0.01 μg/ml 23894633
HFS? Function Assay 0.01 μg/ml 24 h increases the expression of glycosphingolipid synthase genes at 6 h 23894633
OVCAR-3 Growth Inhibition Assay 1–15?μM? 24?h induces a loss of cell viability dose dependently? 23826964
OVCAR-3 Function Assay 1–15?μM? 24?h induces nuclear damage 23826964
OVCAR-3 Apoptosis Assay 1-10?μM 4 h induces the activation of apoptosis-related proteins 23826964
OVCAR-3 Apoptosis Assay 10?μM 24?h induces activation of caspases 23826964
OVCAR-3 Function Assay 1–10?μM 24?h induces disruption of the mitochondrial transmembrane potential 23826964
OVCAR-3 Function Assay 1–10?μM 24?h induces formation of reactive oxygen species 23826964
OVCAR-3 Function Assay 1–10?μM 24?h inhibits cell adhesion and migration 23826964
HeLa Function assay 100 uM 2 hrs Dispersion of cis golgi marker betaCoP in human HeLa cells at 100 uM for 2 hrs 17563369
HeLa Function assay 100 uM 2 hrs Dispersion of cis golgi marker KDEL in human HeLa cells at 100 uM for 2 hrs 17563369
Vero Function assay 10 ug/ml 5 mins Inhibition of Arf1 in african green monkey Vero cells assessed as rapid AP-1 dispersal from golgi membranes at 10 ug/ml after 5 mins by immunofluorescence method 19182783
Vero Function assay 10 ug/ml 5 mins Inhibition of Arf1 in african green monkey Vero cells assessed as rapid GGA3 dispersal from trans golgi network at 10 ug/ml after 5 mins by immunofluorescence method 19182783
Vero Function assay 10 uM 1 hr Inhibition of GBF1 QNV deleted mutant in african green monkey Vero cells assessed as effect on change in golgi morphology at 10 uM after 1 hr by immunofluorescence method 19182783
Vero Function assay 10 uM 1 hr Inhibition of GBF1 QNV to AAA mutant in african green monkey Vero cells assessed as effect on change in golgi morphology at 10 uM after 1 hr by immunofluorescence method 19182783
Vero Function assay 10 ug/ml 1 hr Inhibition of Arf1 in african green monkey Vero cells assessed as decrease in Arf1-GTP levels at 10 ug/ml after 1 hr 19182783
Vero Function assay 10 uM 1 hr Inhibition of GBF1 in african green monkey Vero cells assessed as punctate and diffuse distribution of medial-Golgi marker giantin from TGN at 10 uM after 1 hr by immunofluorescence method 19182783
Vero Function assay 10 ug/ml 1 hr Inhibition of Arf1 in african green monkey Vero cells assessed as punctate and diffuse distribution of medial-Golgi marker giantin at 10 ug/ml after 1 hr by immunofluorescence method 19182783
Vero Function assay 10 ug/ml 5 mins Inhibition of Arf1 in african green monkey Vero cells assessed as rapid COPI redistribution from golgi at 10 ug/ml after 5 mins by immunofluorescence method 19182783
Vero Function assay 10 ug/ml 1 hr Inhibition of Arf1 in african green monkey Vero cells assessed as tubule formation from trans golgi network and endosomes before its dispersal at 10 ug/ml after 1 hr by immunofluorescence method 19182783
Vero Function assay 10 ug/ml 1 hr Inhibition of Arf1 in african green monkey Vero cells assessed as giantin positive punctate structures in contact with Sec31-positive ER exit site at 10 ug/ml after 1 hr by immunofluorescence method 19182783
Vero Function assay 10 uM 1 hr Induction of GBF1 in african green monkey Vero cells assessed as punctate and diffuse distribution of cis-Golgi marker GM130 from TGN at 10 uM after 1 hr by immunofluorescence method 19182783
Vero Function assay 10 ug/ml 1 hr Inhibition of Arf1 in african green monkey Vero cells assessed as punctate and diffuse distribution of cis-Golgi marker GM130 at 10 ug/ml after 1 hr by immunofluorescence method 19182783
NRK Function assay 7 uM 60 mins Golgi-disturbing activity in golgi apparatus of rat NRK cells assessed as fusion of golgi membrane fusion with endoplasmic reticulum at 7 uM after 60 mins by Hoechst 3342 staining-based immunofluorescence microscopy 20189813
HeLa R19 Antiviral assay 0.5 uM 7 hrs Antiviral activity against Coxsackievirus B3 infected in human HeLa R19 cells assessed as inhibition of viral replication at 0.5 uM after 7 hrs by luciferase reporter gene assay 23805957
HeLa Function assay 5 uM 30 to 60 mins Induction of golgi apparatus disassembly in human HeLa cells at 5 uM after 30 to 60 mins by confocal microscopic analysis 23805957
Arabidopsis thaliana root cells Function assay 90 uM 30 mins Induction of morphological changes of golgi apparatus in Arabidopsis thaliana root cells expressing ST-YFP/VHAa1-RFP at 90 uM after 30 mins by confocal laser scanning microscopic analysis 23805957
HeLa R19 Antiviral assay 5 to 50 uM 7 hrs Antiviral activity against Coxsackievirus B3 infected in human HeLa R19 cells assessed as inhibition of viral replication at 5 to 50 uM after 7 hrs by luciferase reporter gene assay 23805957
PC3 Function assay 50 nM 72 hrs Potentiation of 3 nM docetaxel-induced cytotoxicity against human PC3 cells assessed as decrease in cell viability at 50 nM after 72 hrs by trypan blue exclusion assay 28462831
HeLa Function assay 18 uM 3 hrs Inhibition of alkaline phosphatase secretion in human HeLa cells at 18 uM incubated for 3 hrs 31421965
PRP Function Assay 10 μM abrogates SDF-1α-mediated CXCR7 externalization? 24668750
Vero Function assay 10 uM Inhibition of GBF1 in african green monkey Vero cells assessed as inhibition of StxB-SS retrogade transport from endosomes to TGN at 10 uM by immunofluorescence method 19182783
Vero Function assay 10 ug/ml Inhibition of Arf1 in african green monkey Vero cells assessed as inhibition of StxB-SS retrogade transport from endosomes to TGN at 10 ug/ml by immunofluorescence method 19182783
L02 Cytotoxicity assay 72 hrs Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50<0.0004μM. 28494251
PC3 Antiproliferative assay 72 hrs Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay, IC50=0.068μM. 28494251
HT-29 Antiproliferative assay 72 hrs Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay, IC50=0.16μM. 28494251
HepG2 Antiproliferative assay 72 hrs Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay, IC50=0.35μM. 28494251
LO2 Antiproliferative assay 72 hrs Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay, IC50<0.001μM. 29524728
Bel7402 Antiproliferative assay 72 hrs Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay, IC50=0.024μM. 29524728
HL60 Antiproliferative assay 72 hrs Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, IC50=0.025μM. 29524728
PC3 Antiproliferative assay 72 hrs Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay, IC50=0.068μM. 29524728
Bel7402/5-FU Antiproliferative assay 72 hrs Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay, IC50=0.82μM. 29524728
VERO-E6 Function assay 48 hrs Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of VERO-E6 cells after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging, IC50=0.02μM. ChEMBL
VERO-E6 Function assay 48 hrs Toxicity CC50 against VERO-E6 cells determined at 48 hours by high content imaging (same conditions as 2_LEY without exposure to 0.01 MOI SARS CoV-2 virus), CC50=0.06μM. ChEMBL
MKN45 Growth Inhibition Assay IC50<0.001 μg/ml 23793342
LOVO Growth Inhibition Assay IC50=0.12 μg/ml 23793342
A549 Growth Inhibition Assay IC50=0.04 μg/ml 23793342
MDA-MB-435 Growth Inhibition Assay IC50<0.001 μg/ml 23793342
HepG2 Growth Inhibition Assay IC50<0.001 μg/ml 23793342
HL-60 Growth Inhibition Assay IC50<0.001 μg/ml 23793342
neural precursor cells Function assay Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay 17417631
NCI60 Cytostatic assay Cytostatic activity against human NCI60 cells by SRB assay, GI50=0.0206μM. 23805957
NCI60 Cytostatic assay Cytostatic activity against human NCI60 cells by SRB assay, TGI=3.48μM. 23805957
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生物活性

產(chǎn)品描述 Brefeldin A (BFA)作用于HCT 116細(xì)胞，抑制內(nèi)酯抗生素和ATPase，作用于protein transport（蛋白轉(zhuǎn)運(yùn)），IC50為0.2 μM，誘導(dǎo)癌細(xì)胞分化和凋亡。它還能提高同源重組修復(fù)效率，是CRISPR-mediated HDR的增強(qiáng)劑。Brefeldin A 也是自噬和線粒體自噬的抑制劑。
靶點(diǎn)
ATPase (HCT 116) [1]
0.2 μM
體外研究(In Vitro)
體外研究活性

Brefeldin A是一種真菌代謝產(chǎn)物，抑制內(nèi)質(zhì)網(wǎng)和高爾基體之間的傳輸，Brefeldin A導(dǎo)致膜蛋白分布受損。Brefeldin A處理HCT 116人結(jié)腸癌細(xì)胞，觀察到形態(tài)學(xué)的變化，表示細(xì)胞分化。Brefeldin A作用于腫瘤細(xì)胞，主要通過誘導(dǎo)分化和凋亡而發(fā)揮其細(xì)胞毒性作用。[1]

20 μg/mL Brefeldin A處理檢測(cè)條6小時(shí)，在10mM Indomethacin 和 30 μM L-NOARG存在時(shí)，完全廢除Bradykinin誘導(dǎo)的松弛。濃度為1 nM 到 1 mM之間的 20 μg/mL Brefeldin A處理，廢除Bradykinin誘導(dǎo)的[Ca2+]i降低。Brefeldin A作用于內(nèi)皮細(xì)胞，對(duì)Bradykinin 或 Substance P誘導(dǎo)的[Ca2+]i 提高沒有影響。[2]

Brefeldin A 不影響GTPS與myr-rARF1的自發(fā)磷脂依賴性的結(jié)合，但完全廢除視網(wǎng)膜等滲提取物（RIE）的催化交換，2 μM Brefeldin A的半抑制濃度為2 μM。Brefeldin A 抑制多種膜轉(zhuǎn)運(yùn)途徑。Brefeldin A作用于高爾基體膜或腦細(xì)胞質(zhì)，抑制ADP-核糖基化因子特定的鳥嘌呤核苷酸交換活性。Brefeldin A完全抑制說明視網(wǎng)膜提取物包含ARF特定的鳥嘌呤核苷酸交換因子。Brefeldin A只部分抑制ADP-核糖基化因子（ARFs）釋放的視網(wǎng)膜等滲提取物（RIE）催化的GTPS，即使?jié)舛雀哌_(dá)300 μM時(shí)。[3]

Brefeldin A誘導(dǎo)高爾基體與ER融合。Brefeldin A廢除 CERT 抑制劑HPA-12的抑制效果。Brefeldin A處理CHO細(xì)胞，使鞘磷脂的合成提高2到3倍。[4]

除了B-CLL細(xì)胞，Brefeldin A還造成多發(fā)性骨髓瘤（U266，NCI-H929），JURKAT，HeLa細(xì)胞，白血病（HL60，K562，BJAB），結(jié)腸（HT-29），和前列腺，及腺樣囊性瘤細(xì)胞發(fā)生凋亡。25 ng/mL Brefeldin A處理 HF4.9 和 HF28RA 細(xì)胞，完全抑制細(xì)胞生長，而要完全抑制HF1A3 細(xì)胞生長則需要高劑量的Brefeldin A (75 ng/mL) 。50-75 ng/mL Brefeldin A 處理HF1A3, HF4.9 和 HF28RA細(xì)胞，24小時(shí)內(nèi)抑制細(xì)胞增殖，這種作用存在劑量依賴性，3H-胸甘的滲透幾乎完全停止，50 ng/ml處理時(shí)，HF1A3, HF4.9 和 HF28RA細(xì)胞分別抑制26%, 76%, 87%，75 ng/mL處理時(shí)，HF1A3, HF4.9 和 HF28RA細(xì)胞分別抑制75%, 87%, 92%。YO-PRO 1/PI檢測(cè)中，Brefeldin A誘導(dǎo)細(xì)胞死亡，這種作用存在劑量依賴性。[5]

Brefeldin A可提高同源重組效率，是CRISPR-mediated HDR的增強(qiáng)劑[5]。
細(xì)胞實(shí)驗(yàn) 細(xì)胞系 人類濾泡性淋巴瘤細(xì)胞系HF1A3，HF4.9 和HF28RA
濃度 0 ng/mL-75 ng/mL
孵育時(shí)間 5 天
方法

使用 YO-PRO 1/PI 和 SYTO16/PI 探針對(duì)細(xì)胞進(jìn)行雙染色，測(cè)定HF1A3, HF4.9細(xì)胞活力。為了測(cè)定細(xì)胞增殖使用0–100 ng/mL Brefeldin A 在完全培養(yǎng)基中處理細(xì)胞20小時(shí)，然后加入1 μCi/mL [methyl-3H]-胸甘在37°C下再處理4小時(shí)。使用 Microbeta 計(jì)數(shù)儀對(duì)滲透的放射性胸甘進(jìn)行閃爍計(jì)數(shù)。為了測(cè)定Brefeldin A治療的長期效果，細(xì)胞按初始濃度105 cells/mL接種，然后使用0-75 ng/mL Brefeldin A處理長達(dá)5天。在指定時(shí)間，移除細(xì)胞樣本，通過標(biāo)準(zhǔn)臺(tái)酚藍(lán)排除法測(cè)定存活細(xì)胞數(shù)。
實(shí)驗(yàn)圖片 檢測(cè)方法 檢測(cè)指標(biāo) 實(shí)驗(yàn)圖片 PMID
Western blot p53 / GRP78 22859938
Immunofluorescence MTP / GBF1 ErbB3 / Calnexin FMNL1 / GM130 26267806
Growth inhibition assay Cell viability 28462831

化學(xué)信息&溶解度

分子量 280.36 分子式

C16H24O4
CAS號(hào) 20350-15-6 SDF Download Brefeldin A (BFA) SDF
Smiles CC1CCCC=CC2CC(CC2C(C=CC(=O)O1)O)O
儲(chǔ)存條件(自收到貨起)

體外溶解度
批次：

DMSO : 56 mg/mL ( (199.74 mM) ；DMSO吸濕會(huì)降低化合物溶解度，請(qǐng)使用新開封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩爾濃度計(jì)算器

體內(nèi)溶解度
批次：

現(xiàn)配現(xiàn)用，請(qǐng)按從左到右的順序依次添加，澄清后再加入下一溶劑

 動(dòng)物體內(nèi)配方計(jì)算器

實(shí)驗(yàn)計(jì)算

摩爾濃度計(jì)算器

質(zhì)量 濃度 體積 分子量

動(dòng)物體內(nèi)配方計(jì)算器（澄清溶液）

第一步：請(qǐng)輸入基本實(shí)驗(yàn)信息（考慮到實(shí)驗(yàn)過程中的損耗，建議多配一只動(dòng)物的藥量）

mg/kg g μL 只

第二步：請(qǐng)輸入動(dòng)物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請(qǐng)聯(lián)系Selleck為您提供正確的澄清溶液配方）

% DMSO % % Tween 80 % ddH2O
%DMSO %

計(jì)算結(jié)果：

工作液濃度： mg/ml；

DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請(qǐng)先聯(lián)系Selleck）；

體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH2O，混勻澄清。

體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。

注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。

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