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  1. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  2. Reactive Oxygen Species
  3. MCLA hydrochloride

MCLA hydrochloride 是一種化學(xué)發(fā)光試劑,可用于定量超氧化物的含水量。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報,我們不為任何個人用途提供產(chǎn)品和服務(wù)

MCLA hydrochloride Chemical Structure

MCLA hydrochloride Chemical Structure

CAS No. : 128322-44-1

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3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
1 mg ¥1200
In-stock
5 mg ¥2450
In-stock
10 mg   詢價  
50 mg   詢價  

* Please select Quantity before adding items.

Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

MCLA hydrochloride is a chemiluminescent reagent which can be used to quantify aqueous concentrations of superoxide.

體外研究
(In Vitro)

The non-specific luminescence remains almost constant for 10 min after the addition of MCLA hydrochloride (MCLA) and is not significantly influenced by SOD. The MCLA hydrochloride method is, however, 4.5-times more sensitive than the CLA method[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

291.73

Formula

C14H14ClN3O2

CAS 號
性狀

固體

顏色

Light yellow to yellow

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性數(shù)據(jù)
細胞實驗: 

DMSO 中的溶解度 : 10 mg/mL (34.28 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響,請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.4278 mL 17.1391 mL 34.2783 mL
5 mM 0.6856 mL 3.4278 mL 6.8557 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C儲存時,請在6個月內(nèi)使用,-20°C儲存時,請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息:

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

由于實驗過程有損耗,建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

純度: 99.81%

參考文獻
Kinase Assay
[1]

The standard reaction mixture contains 1×l0-7 M MCLA hydrochloride (MCLA), 5×10-5 M hypoxanthine, XOD (6.5 U), SOD (0.2 to 20 ng/mL) or none, and 50 mM Tris-HCl buffer containing 0.1 mM EDTA at pH 7.8, in a total volume of 3.0 mL. Chemiluminescence measurement is initiated by the addition of MCLA hydrochloride to the standard incubation mixture excluding XOD, continued for 4 min without additive and for an additional 4 min after the addition of XOD. Chemiluminescence is measured using a luminescence reader at 25°C[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C儲存時,請在6個月內(nèi)使用,-20°C儲存時,請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4278 mL 17.1391 mL 34.2783 mL 85.6957 mL
5 mM 0.6856 mL 3.4278 mL 6.8557 mL 17.1391 mL
10 mM 0.3428 mL 1.7139 mL 3.4278 mL 8.5696 mL
15 mM 0.2285 mL 1.1426 mL 2.2852 mL 5.7130 mL
20 mM 0.1714 mL 0.8570 mL 1.7139 mL 4.2848 mL
25 mM 0.1371 mL 0.6856 mL 1.3711 mL 3.4278 mL
30 mM 0.1143 mL 0.5713 mL 1.1426 mL 2.8565 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

產(chǎn)品名稱:
MCLA hydrochloride
目錄號:
HY-W013275
需求量: