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  2. Immunology/Inflammation
  3. SOD
  4. Antioxidant peptide A

Antioxidant peptide A 是一種短肽。作用于癌細(xì)胞時，Antioxidant peptide A 的側(cè)鏈有助于增強(qiáng)自由基清除活性。

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Custom Peptide Synthesis

Antioxidant peptide A Chemical Structure

Antioxidant peptide A Chemical Structure

CAS No. : 159147-88-3

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Antioxidant peptide A 的其他形式現(xiàn)貨產(chǎn)品:

Customer Review

Other Forms of Antioxidant peptide A:

  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Antioxidant peptide A is a short peptide, which contains alternative aromatic or sulfur-containing amino acid. The side chains of Antioxidant peptide A are believed to contribute to strong radical scavenging activities of peptides in the cancer cell.

體外研究
(In Vitro)

The effects of 10-100 μM of Antioxidant peptide A (Pep-A) concentrations are studied on the superoxide dismutase (SOD) enzyme activity. The enzyme activity decreases by 0.5 and 0.7-folds at 10 and 50 μM Antioxidant peptide A concentrations, respectively, and increases by 1.79-folds at 100 μM Antioxidant peptide A treatment, indicating that this concentration can be ideal for the treatment on Y79 a, RB cells. Furthermore, the Antioxidant peptide A can be involved in decreasing the ROS by increasing the antioxidant enzyme activity. A similar increase in the antioxidative enzyme levels in the presence of Hoki skin antioxidative peptide in hepatocarcinoma cells is attributed to the peptide’s role in maintaining the redox balance in the cellular environment. Cell viability analysis results show that the Antioxidant peptide A shows no toxicity to cancerous (Y79) cells and non-cancerous cells even after 48 h of treatment. The Y79 RB cell viability ranges between 115 and 157 % and 111-126 % after 24 and 48 h of exposures with Antioxidant peptide A, respectively. The cancer cell death from the treatment of 10-100 μM GNPs concentration is studied[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

770.97

Formula

C31H54N12O7S2
CAS 號
Sequence

Pro-His-Cys-Lys-Arg-Met
Sequence Shortening

PHCKRM
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
細(xì)胞實(shí)驗(yàn)： 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
純度 & 產(chǎn)品資料
參考文獻(xiàn)
Kinase Assay
[1]

Y79 cells are seeded at 2×105 cells/well in 12-well plates with 1000 μL of culture media and incubated at 37°C overnight. The cells are then exposed to varying dosages of Antioxidant peptide A (Pep-A) and Pep-B (10, 50, and 100 μM) in fresh medium and then incubated for 6 h. At the end of the incubation, the cells are collected and washed with ice-cold PBS twice and lysed with cell lysis buffer [0.1 M Tris/HCl, pH 7.4 containing 0.5 % Triton X-100, 5 mM β-Mercaptoethanol, 0.1 mg/mL PMSF]. Cell lysate is centrifuged at 14000×g for 5 min at 4°C. The supernatant contains a combined SOD activity from cytosol and mitochondria. The SOD activity is measured using the superoxide dismutase (SOD) activity assay kit. In brief, the cell lysate, buffer, enzyme, and the WST reagent are diluted, and the solution is added as per the protocol and incubated at 37°C for 20 min and read at 450 nm on a microplate reader. SOD activity is then calculated as a percentage of the inhibition activity of xanthine oxidase[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[1]

Y79 retinoblastoma and MIOM1 cells are seeded at 5×103 cells/well in 96-well plates and incubated at 37°C overnight. The cells are then treated with varying concentrations (10, 30, 60, and 100 μM) of Antioxidant peptide A (Pep-A) and Pep-B in a fresh medium and incubated for specific time periods (24 and 48 h). At the end of the incubation, 10 μL of MTT (5 mg/mL) is added to the cells with fresh medium (100 μL) and incubated at 37°C until formazan crystals are formed. The formazan crystals are dissolved in 100 μL of DMSO, and the reading is taken at 570 nm. Cell viability is calculated[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Antioxidant peptide A
目錄號：
HY-P1512
需求量：