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  1. Anti-infection Apoptosis Immunology/Inflammation TGF-beta/Smad
  2. Bacterial TNF Receptor Interleukin Related TGF-β Receptor
  3. Lysozyme

Lysozyme  (Synonyms: 溶菌酶; Muramidase)

目錄號: HY-P1068 純度: ≥95.0%
COA 產(chǎn)品使用指南

Lysozyme (Muramidase) 是一種保守的抗微生物蛋白。Lysozyme 通過水解細菌細胞壁肽聚糖 (PG) 來發(fā)揮殺菌作用。Lysozyme 在限制粘膜表面和其他部位的細菌生長方面起著重要作用,它不僅可以控制潛在的致病菌,還可以限制微生物群的過度生長以防止生態(tài)失調(diào)。胞外 Lysozyme 還可將多聚 PG 降解為可溶片段,激活粘膜上皮細胞中的 NOD 受體,導致中性粒細胞和巨噬細胞分泌趨化因子和激活因子。

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Lysozyme Chemical Structure

Lysozyme Chemical Structure

CAS No. : 9001-63-2

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3.  試用裝只面向終端客戶

規(guī)格 價格 是否有貨 數(shù)量
500 μg ¥1200
In-stock
1 mg ¥2100
In-stock
5 mg 現(xiàn)貨 詢價
10 mg   詢價  
50 mg   詢價  

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  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Lysozyme (Muramidase) is a conserved antimicrobial protein. Lysozyme exerts its bactericidal effect by hydrolyzing bacterial cell wall peptidoglycan (PG). Lysozyme plays an important role in limiting bacterial growth on mucosal surfaces and other sites, not only controlling potential pathogens but also limiting overgrowth of microbiota to prevent dysbiosis. Extracellular lysozyme can also degrade polymeric PG into soluble fragments, activate NOD receptors in mucosal epithelial cells, and lead to the secretion of chemokines and activating factors by neutrophils and macrophages[1][2].

體外研究
(In Vitro)

使用說明
使用 10 mM Tris-HCl (pH 8.0) 配制 1 mg/mL 母液,也可用純水溶解,2-8℃ 可儲存一周,避免反復凍融。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

溶菌酶 M 缺陷小鼠 (lysozyme M-/-) 增加了對肺炎克雷伯菌感染的易感性,而溶菌酶表達的增加的小鼠 (lysozymetg) 則提高了對感染的抵抗力并提高了生存率[3]。
Hen Egg Lysozyme (150 mg/kg; i.g.; 每天 1 次持續(xù) 5 天) 減輕了右旋糖酐硫酸鈉 (DSS) 誘導的結(jié)腸炎仔豬模型中炎癥的程度并調(diào)節(jié)局部基因表達[4]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DSS-induced colitis piglet model
Dosage: 150 mg/kg (Hen Egg Lysozyme)
Administration: Once a day for 5 days; gavage
Result: Reduced the expression of local pro-inflammatory cytokines TNF-R, IL-6, IFN-γ, IL-8, and IL-17, while increasing the expression of anti-inflammatory mediators IL-4 and TGF-β.
Clinical Trial
CAS 號
性狀

固體

顏色

White to off-white

中文名稱

溶菌酶

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

溶解性數(shù)據(jù)
細胞實驗: 

H2O 中的溶解度 : 4.76 mg/mL (ultrasonic and adjust pH to 4 with 0.1 M HCL)

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息:

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

由于實驗過程有損耗,建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度,如有需要,請與 MCE 中國技術支持聯(lián)系。
純度 & 產(chǎn)品資料

純度: ≥95.0%

參考文獻
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱:
Lysozyme
目錄號:
HY-P1068
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