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  2. Anti-infection
  3. Bacterial
  4. Indolicidin

Indolicidin是從牛嗜中性粒細(xì)胞的細(xì)胞質(zhì)顆粒中純化的有效的抗微生物肽。

在相同的摩爾濃度下，化合物鹽形式與游離形式有相同的生物活性，但鹽形式 Indolicidin acetate 通常具有更好的水溶性和穩(wěn)定性。

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Custom Peptide Synthesis

Indolicidin Chemical Structure

Indolicidin Chemical Structure

CAS No. : 140896-21-5

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Indolicidin 的其他形式現(xiàn)貨產(chǎn)品:

Customer Review

Other Forms of Indolicidin:

  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Indolicidin is a potent antimicrobial peptide purified from the cytoplasmic granules of bovine neutrophils.

體外研究
(In Vitro)

Indolicidin is comprised of 13 amino acids, 5 of which are tryptophan residues, and the carboxylterminal arginine is carboxamidated. Indolicidin has the highest tryptophan content of any known protein. The multiple tryptophan residues may play an important role in the function of this unique antibiotic peptide. Indolicidin is a tridecapeptide amide which possesses in vitro bactericidal activities comparable with the most active of the defensin or bactenecin peptides[1]. Indolicidin binds purified surface lipopolysaccharide with high affinity and permeabilized the outer membrane of Escherichia coli to the small hydrophobic molecule 1-N-phenylnapthylamine (Mr 200), results consistent with indolicidin crossing the outer membrane via the self-promoted uptake pathway. The methyl esterification of indolicidin's carboxyl terminus increases its activity for Gram-negative and Gram-positive bacteria. In Gram-negative bacteria this is associated with an increased binding to lipopolysaccharide and increased permeabilization of the outer membrane. The cytoplasmic membrane is the site of action of indolicidin as assayed in Escherichia coli by the unmasking of cytoplasmic beta-galactosidase due to membrane permeabilization[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1906.28

Formula

C100H132N26O13
CAS 號
性狀

固體

顏色

White to off-white

Sequence

Ile-Leu-Pro-Trp-Lys-Trp-Pro-Trp-Trp-Pro-Trp-Arg-Arg-NH2
Sequence Shortening

ILPWKWPWWPWRR-NH2
結(jié)構(gòu)分類
初始來源

bovine neutrophil
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

純度 & 產(chǎn)品資料
參考文獻(xiàn)
Cell Assay
[1]

The time course and dose dependence of indolicidin bactericidal activity are determined using Escherichia coli ML35 and Staphylococcus aureus 502A as test organisms. The assays are performed in 10 mM sodium phosphate buffer, pH 7.4, at 37°C. A 50 μg sample of indolicidin is dissolved in 50 pl of 0.1 M pyridine acetate, pH 6.5, and incubated with 5 μg of chymotrypsin for 15 h at 37°C. Digestion of the sample is confirmed by the disappearance of the indolicidin band on acid-urea PAGE. Following lyophilization, untreated and digested samples are tested for antibacterial activity in a agar diffusion assay against Escherichia coli using concentrations of peptide ranging from 10 to 300 μg/mL[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Indolicidin
目錄號：
HY-P0261
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