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  2. 多肽產(chǎn)品
  3. 多肽及其衍生物
  4. 多肽藥物遞送
  5. 細胞穿膜肽
  6. (Arg)9 TFA

(Arg)9 TFA  (Synonyms: Nona-L-arginine TFA; Peptide R9 TFA)

目錄號: HY-P0133A
產(chǎn)品使用指南 技術(shù)支持

(Arg)9 (Nona-L-arginine) TFA 是一種由 9 個精氨酸殘基組成的細胞穿透肽 (CPP)。(Arg)9 TFA 具有神經(jīng)保護功能，在谷氨酸模型中 IC50 值為 0.78 μM。

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Custom Peptide Synthesis

(Arg)9 TFA Chemical Structure

(Arg)9 TFA Chemical Structure

CAS No. : 2283335-13-5

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(Arg)9 TFA 的其他形式現(xiàn)貨產(chǎn)品:

Customer Review

Other Forms of (Arg)9 TFA:

MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

(Arg)9 (Nona-L-arginine) TFA is a cell-penetrating peptide (CPP) made up of 9 arginine residues. (Arg)9 TFA has neuroprotective property, exhibits neuroprotective activity with an IC50 of 0.78 μM in the glutamic acid model[1][2].

IC50 & Target

IC50: 0.78 μM (neuroprotection)[1].
體外研究
(In Vitro)

(Arg)9 (Nona-L-arginine; 5-10 μM) TFA provides significant neuroprotection in a dose–response manner following glutamic acid exposure (IC50=0.78 μM). Following kainic acid exposure, (Arg)9 TFA is neuroprotective, but less effective than in the glutamic acid model (IC50=0.81 μM). (Arg)9 TFA also shows neuroprotection following in vitro ischemia (IC50=6 μM)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

(Arg)9 (Nona-L-arginine; 1?μM/kg (600? μL); i.v.; once, for 30min; male Sprague–Dawley ratspermanent middle cerebral artery stroke model) TFA shows neuroprotective effects and reduces infarct volume[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague–Dawley rats (270 to 320?g) permanent middle cerebral artery stroke model[2]
Dosage: 1 μM/kg (600? μL)
Administration: Intravenous injection; once, over 5 minutes
Result: Reduced significantiy 20% in infarct volume.
分子量

1423.69 (free acid)

Formula

C54H110N36O10.xC2HF3O2
CAS 號
性狀

固體

顏色

White to off-white

Sequence

Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg
Sequence Shortening

RRRRRRRRR
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
細胞實驗： 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
純度 & 產(chǎn)品資料
參考文獻

  • 摩爾計算器

  • 稀釋計算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
(Arg)9 TFA
目錄號：
HY-P0133A
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