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NEP (VDP-green (NEP)) 是一種基于分子內(nèi)電荷轉(zhuǎn)移 (ICT) 機制的感應熒光探針，用于感應含鄰二硫醇的蛋白質(zhì) (VDPs)。NEP 在活細胞和動物體內(nèi)對 VDPs 表現(xiàn)出高選擇性，并顯示強綠色熒光信號 (λex/λem=430/535 nm)。NEP 具有用于帕金森氏癥研究的潛力。

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NEP Chemical Structure

NEP Chemical Structure

CAS No. : 2414276-32-5

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  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

NEP (VDP-green (NEP)) is a turn-on fluorescent probe based on the intramolecular charge transfer (ICT) mechanism for sensing vicinal dithiol-containing proteins (VDPs). NEP exhibits high selectivity toward VDPs in live cells and in vivo and displays a strong green fluorescence signal (λex/λem=430/535 nm). NEP has the potential for parkinsonism[1].

體外研究
(In Vitro)

NEP (1-30 μM; 6 hours) has no or little cytotoxicity in HepG2 and PC12 cells[1].
NEP (10 μM; 4 hours) causes the fluorescence intensity to decrease gradually in PC12 cells pretreated with 6-OHDA (0, 50, 100, and 200 μM; for 30 min)[1].
NEP contains a dithiarsolane moiety (five-membered As-S ring) as the receptor of VDPs. In the presence of VDPs, NEP displays a strong green fluorescence signal produced by the cyclic dithiarsolane cleavage and subsequent intramolecular cyclization to liberate the fluorophore. NEP maintains a reliable fluorescence response within the range of pH 7-10[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

NEP (10 μM; for 4 h) causes the obvious green signal in zebrafishes (4 day old)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

654.63

Formula

C29H31AsN4O5S2
CAS 號
Emission (Em)

535
Excitation (Ex)

430
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
純度 & 產(chǎn)品資料
參考文獻

  • 摩爾計算器

  • 稀釋計算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
NEP
目錄號：
HY-D1259
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