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  2. Biochemical Assay Reagents
  3. C-Phycocyanin

C-Phycocyanin  (Synonyms: C-藻藍(lán)蛋白; C-PC)

目錄號: HY-D1025
COA 產(chǎn)品使用指南

C-phycocyanin (C-PC) 是一種蛋白質(zhì)色素,也廣泛用作人類的優(yōu)良營養(yǎng)補(bǔ)充劑。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào),我們不為任何個人用途提供產(chǎn)品和服務(wù)

C-Phycocyanin Chemical Structure

C-Phycocyanin Chemical Structure

CAS No. : 11016-15-2

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Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻(xiàn)

  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

C-phycocyanin (C-PC) is a protein pigment which is also widely used as an excellent nutrient supplement for human beings.

體外研究
(In Vitro)

對于組合實(shí)驗(yàn),全反式維甲酸 (ATRA) 聯(lián)合 C-Phycocyanin 處理細(xì)胞時(shí),IC50 值低于ATRA 組。但在相同 IC50 下,C-phycocyanin 用量越多,ATRA 用量越少。結(jié)果表明,C-Phycocyanin 聯(lián)合 ATRA可顯著降低 CDK-4 mRNA 水平 (P<0.05)。C-Phycocyanin +ATRA 聯(lián)合組的聯(lián)合指數(shù) (CI) 值為 0.852,小于 1,表明在 88 μg/L C-Phycocyanin +0.102 mM ATRA 的作用濃度下,具有協(xié)同作用。與對照組相比,C-Phycocyanin 或 ATRA 處理組的綜合光密度 (IOD) 增加,差異有統(tǒng)計(jì)學(xué)意義 (P<0.05),聯(lián)合組差異進(jìn)一步擴(kuò)大 (P<0.01)。C-Phycocyanin 組 caspase-3 的表達(dá)高于對照組,但與 ATRA 組相似,而聯(lián)合組的表達(dá)量最大[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

與對照組相比,單獨(dú) C-Phycocyanin 或 ATRA 處理組的小鼠平均腫瘤重量顯著降低,C-Phycocyanin +ATRA 協(xié)同組進(jìn)一步降低。結(jié)果表明,單一的 C-Phycocyanin 可以增加小鼠的脾臟重量,而 ATRA 的作用恰恰相反。C-Phycocyanin 還可以促進(jìn)小鼠免疫器官的生長,提高機(jī)體的免疫功能。C-Phycocyanin 與 ATRA 單獨(dú)使用可顯著抑制 Cyclin D1的表達(dá),聯(lián)合使用時(shí)抑制效果更明顯[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 號
性狀

液體

顏色

Light blue to blue

中文名稱

C-藻藍(lán)蛋白;C-藻藍(lán)素

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

純度 & 產(chǎn)品資料
參考文獻(xiàn)
Cell Assay
[1]

A549 cells are divided into four groups: (1) control group: the cells are not treated with any drug. (2) C-phycocyanin (C-PC) treated group: the cells are treated with C-phycocyanin alone. (3) All-trans retinoic acid (ATRA) treated group: the cells were treated with ATRA alone. (4) C-phycocyanin+ATRA combined group: the cells are treated simultaneously with C-phycocyanin and ATRA. For the combined treatment group, the combination index (CI) between two drugs is calculated. CI values of less than, equal to, and more than 1 indicate synergy, additivity, and antagonism, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

A total of 40 adult NU/NU mice (20 males and 20 females, 20 to 22 g) are used for the study. The mouse tumor models are set up by subcutaneous injection of 2×106 A549 cells near the armpit area. Fifteen days later, the mice are randomly divided into four groups: control group, All-trans retinoic acid (ATRA) treatment group which is treated with 0.2 mL ATRA (10 mM), C-phycocyanin treatment group which is given 0.2 mL C-phycocyanin (320 mg/mL), and C-phycocyanin+ATRA treatment group receiving 0.2 mL C-phycocyanin (320 mg/mL) and 0.2 mL ATRA (10 mM) at the same time. These agents are injected into the area of tumors and the duration of drug treatment is 10 days. Two days after drug withdrawal, the mice are executed, and then tumors and spleens are picked out[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻(xiàn)
  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱:
C-Phycocyanin
目錄號:
HY-D1025
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