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  1. Metabolic Enzyme/Protease
  2. Phospholipase MAGL
  3. 7-Hydroxycoumarinyl arachidonate

7-Hydroxycoumarinyl arachidonate  (Synonyms: 7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate)

目錄號(hào): HY-116141 純度: ≥98.0%
COA 產(chǎn)品使用指南

7-Hydroxycoumarinyl arachidonate (7-HCA) 是胞質(zhì)磷脂酶 A2 (PLA2) 的熒光底物,也是單?;视椭久?(MAGL) 的熒光底物。MAGL 蛋白催化 7-Hydroxycoumarinyl arachidonate 的水解,生成花生四烯酸 (AA;HY-109590) 和高熒光的 7-羥基香豆素 (7-HC;HY-N0573),7-HC 的釋放可以用熒光計(jì)進(jìn)行測(cè)量。

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7-Hydroxycoumarinyl arachidonate Chemical Structure

7-Hydroxycoumarinyl arachidonate Chemical Structure

CAS No. : 161180-11-6

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5 mg (55.73 mM * 200 μl ethanol) ¥1000
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查看 Phospholipase 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer[1][2][3].

體外研究
(In Vitro)

MAGL catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonate (7-HCA) into arachidonic acid (AA) and 7-HC. Release of 7-HC, which is fluorescent, is monitored over time with excitation (Ex) at 355 nm and emission (Em) at 460 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

448.59

Formula

C29H36O4

CAS 號(hào)
性狀

液體

顏色

Colorless to light yellow

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Solution, -20°C, 2 years

純度 & 產(chǎn)品資料

純度: ≥98.0%

參考文獻(xiàn)

7-Hydroxycoumarinyl arachidonate 相關(guān)分類(lèi)

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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  • Do most proteins show cross-species activity?

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產(chǎn)品名稱(chēng):
7-Hydroxycoumarinyl arachidonate
目錄號(hào):
HY-116141
需求量: