Cucurbit[8]uril is a potent, low toxicity and orally active supramolecular inducer of protein heterodimerization. Cucurbit[8]uril induces heterodimerization of methylviologen and naphthalene functionalized proteins. Cucurbit[8]uril can induce energy transfer ^[1][2].

Protein Heterodimerization^[1]

Cucurbit[8]uril (0~20 μM; 48 hours; CHO-K1 cells) makes the relative cell viability dropped marginally to 86%^[2].
Cucurbit[8]uril indeed selectively induces the heterodimerization of MV–eYFP with Np–eCFP. Cucurbit[8]uril-induced high energy transfer between the proteins is only observed in the presence of all three supramolecular components, allowing the formation of the ternary complex. In the presence of Cucurbit[8]uril, the unspecific protein assembly induced by the methylviologen is inhibited. The ternary system of Cucurbit[8]uril with methylviologen (MV) and naphthalene (NP) can also be successfully used for the formation of selective protein heterodimers of more hydrophobic proteins. The presence of Cucurbit[8]uril as a host molecule is required to prevent MV induced unspecific dimerization with hydrophobic protein surfaces^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cucurbit[8]uril shows a very low toxicity of the in vivo intravenous injection, as well as oral administration studies on mice^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1329.10

C₄₈H₄₈N₃₂O₁₆

259886-51-6

固體

White to off-white

葫蘆[8]脲； 葫蘆脲[8]

Room temperature in continental US; may vary elsewhere.

• [1]. Uhlenheuer DA, et al. Cucurbit[8]uril induced heterodimerization of methylviologen and naphthalene functionalized proteins. Chem Commun (Camb). 2011;47(24):6798-6800.  [Content Brief]

  [2]. Uzunova VD, et al. Toxicity of cucurbit[7]uril and cucurbit[8]uril: an exploratory in vitro and in vivo study. Org Biomol Chem. 2010;8(9):2037-2042.  [Content Brief]