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  3. Gelatins

Gelatins  (Synonyms: 明膠)

目錄號(hào): HY-Y1365 純度: ≥98.0%
COA 產(chǎn)品使用指南

Gelatins 是一種源自膠原蛋白部分水解的無(wú)毒、無(wú)致癌、可生物降解、不易刺激的天然聚合物,因其強(qiáng)大的液體吸收和溶脹能力具有優(yōu)異的止血性能,可以作為金屬納米顆粒的還原、生長(zhǎng)和穩(wěn)定性的基質(zhì)材料,也可以用于腫瘤細(xì)胞培養(yǎng)和腫瘤治療。

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Gelatins Chemical Structure

Gelatins Chemical Structure

CAS No. : 9000-70-8

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50 mg ¥322
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100 mg ¥500
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Customer Review

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Gelatins is a non-toxic, non carcinogenic, biodegradable, and non irritating natural polymer derived from partial hydrolysis of collagen. Due to its strong liquid absorption and swelling ability, Gelatins has excellent hemostatic properties and can be used as a matrix material for the reduction, growth, and stability of metal nanoparticles. Gelatins can also be used for tumor cell culture and tumor therapy[1][2][3].

體外研究
(In Vitro)

Gelatins (6-12 %, 5 天) 通過(guò)形成 gelatin@NWF 衍生物作為長(zhǎng)期儲(chǔ)存支架促進(jìn)形成前列腺腫瘤細(xì)胞球體,用于培養(yǎng)腫瘤細(xì)胞[3]。
Gelatins (0-200 μg/mL, 24 h) 可以增強(qiáng) HaCaT 細(xì)胞的細(xì)胞粘附、細(xì)胞生長(zhǎng)和傷口愈合,并保護(hù) HaCaT 細(xì)胞免受H2O2誘導(dǎo)的細(xì)胞損傷[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3].

Cell Line: Human prostate cancer cell DU145
Concentration: 6-12 %
Incubation Time: 5 days
Result: Supported more cell growth than pure non-woven fabric (NWF) scaffolds

Cell Viability Assay[5].

Cell Line: Human keratinocyte-derived (HaCaT) cells
Concentration: 0-200 μg/mL
Incubation Time: 24 h
Result: Increased cell growth percentage in the concentration range of 25-75 μg/mL, but decreased cell growth percentage in the concentration range of 100-200 μg/mL.
體內(nèi)研究
(In Vivo)

Gelatins (20 μg/kg,腹腔注射) 通過(guò)形成金納米顆粒/明膠納米復(fù)合材料對(duì) Wistar 大鼠脊髓損傷具有良好的運(yùn)動(dòng)恢復(fù)改善作用[1]。
Gelatins 在與生物相容性二醛淀粉 (DAS) 交聯(lián)合成胺化明膠海綿后對(duì)大鼠股動(dòng)脈損傷具有優(yōu)異的止血作用[2]。
Gelatins (10 mg DOX/kg; 每 2 天 1 次; 14 天; 靜脈注射) 通過(guò) PEG 修飾形成 pH/酶響應(yīng)復(fù)合物可以特異性遞送阿霉素 (DOX) (HY-15142A),在 S180 異位腫瘤小鼠中具有抗腫瘤活性[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Traumatic spinal cord injury Wistar rat model[1].
Dosage: 20 μg/kg Au NPs/Gelatin nanocomposite
Administration: Intraperitoneal injection (i.p.)
Result: Improved hind limb function, reduced cavity area, increased the number of ventral motor neurons, and demonstrated higher BBB scores and lower delayed responses in sensory tests.
Animal Model: SD female rat femoral injury model[2].
Dosage: A pre-weighed hemostatic sponge
Administration: Apply to the wound
Result: Reduced hemostasis time and blood loss.
Animal Model: Female ICR mouse model of ectopic transplantation of mouse sarcoma cell line S180[4].
Dosage: 10 mg DOX/kg
Administration: Intravenous injection (i.v.); once every two days; 14 days
Result: Reduced tumor volume, prevented tumor cell spread, and lowered cardiac toxicity.
CAS 號(hào)
性狀

固體

顏色

White to yellow

中文名稱

明膠

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn): 

H2O 中的溶解度 : 50 mg/mL (超聲助溶)

DMSO 中的溶解度 : 4.55 mg/mL (超聲助溶 (<60°C); 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響,請(qǐng)使用新開(kāi)封的 DMSO)

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn):

以下溶解方案,請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配,在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比; 如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過(guò)加熱和/或超聲的方式助溶。

  • 方案 一

    請(qǐng)依序添加每種溶劑: PBS

    Solubility: 10 mg/mL; 澄清溶液; 超聲助溶

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息:

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

由于實(shí)驗(yàn)過(guò)程有損耗,建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
該產(chǎn)品水溶性佳,請(qǐng)具體參考實(shí)測(cè) 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度,如有需要,請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
純度 & 產(chǎn)品資料

純度: ≥98.0%

參考文獻(xiàn)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱:
Gelatins
目錄號(hào):
HY-Y1365
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