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  1. Immunology/Inflammation NF-κB
  2. Interleukin Related NF-κB
  3. Fibrinogen (Bovine)

Fibrinogen (Bovine) 是一種選擇性蛋白水解分子,可被凝血酶激活組裝成纖維蛋白凝塊。Fibrinogen 能調(diào)節(jié)內(nèi)皮細(xì)胞中 NF-KB 的激活并上調(diào)炎癥趨化因子 MCP-1MCP-1 的表達(dá)。Fibrinogen 在血液凝固、血栓形成、動(dòng)脈粥樣硬化及靜脈移植物病理發(fā)展中起著關(guān)鍵作用,可用于血液凝固以及血管疾病的研究。

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Fibrinogen (Bovine) Chemical Structure

Fibrinogen (Bovine) Chemical Structure

CAS No. : 9001-32-5

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50 mg ¥322
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100 mg ¥500
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Customer Review

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Fibrinogen (Bovine) is a selective proteolytic molecule that can be activated by thrombin to assemble fibrin clots. Fibrinogen can regulate the activation of NF-KB in endothelial cells and upregulate the expression of inflammatory chemokines MCP-1 and MCP-1. Fibrinogen plays a key role in blood clotting, thrombosis, atherosclerosis and the pathological development of venous grafts, and can be used in the study of blood clotting and vascular diseases[1][2][3].

IC50 & Target

IL-8

 

p50

 

p65

 

c-Rel

 

體外研究
(In Vitro)

Fibrinogen (0.3, 2, 4 mg/mL; 30 分鐘) 可以誘導(dǎo)內(nèi)皮細(xì)胞層對(duì)白蛋白的通透性劑量依賴(lài)性增加能引起內(nèi)皮細(xì)胞間的間隙形成,導(dǎo)致白蛋白泄漏[2]。
Fibrinogen (2, 4 mg/mL; 1, 6, 12, 24 小時(shí)) 通過(guò)內(nèi)皮細(xì)胞單層的泄漏隨時(shí)間和劑量的增加而增加[2]。
Fibrinogen (4 mg/mL; 30 分鐘) 會(huì)導(dǎo)致 F-actin 的形成以及內(nèi)皮細(xì)胞單層的間隙形成呈劑量依賴(lài)性增加[2]
Fibrinogen (2, 4 mg/mL; 30 分鐘) 能夠引發(fā) ERK 磷酸化的劑量依賴(lài)性增加[2]。
Fibrinogen (1000 μg/mL; 4 小時(shí)) 可以調(diào)節(jié)內(nèi)皮細(xì)胞 (HUVEC) 中 NF-κB 的激活 NF-κB,并與 p50,p65,c-Rel 亞單位特異性地結(jié)合[3]。
Fibrinogen (250 μg/mL; 4 小時(shí)) 誘導(dǎo)的 NF-κB 激活是獨(dú)立于多粘菌素B (HY-K1044) (5 μg/mL) 和 IL-1β (HY-P80503) (5 μg/mL) 的[3]。
Fibrinogen (100, 250, 500, 1000 μg/mL; 4 小時(shí)) 能夠特異性地、濃度依賴(lài)性地上調(diào)內(nèi)皮細(xì)胞中 MCP-1 和 IL-8 mRNA 的表達(dá)[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[3]

Cell Line: HUVEC cell
Concentration: 100, 250, 500, 1000 μg/mL
Incubation Time: 4h
Result: Increased the MCP-1 from 1.3 times in the control group with 100 μg/mL to 3.1 times at 1000 μg/mL.
Increases IL-8 from 1.2 times in the control group with 100 μg/mL to 2.6 times at 1000 μg/mL.
CAS 號(hào)
性狀

固體

顏色

White to off-white

中文名稱(chēng)

纖維蛋白原(牛)

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

純度 & 產(chǎn)品資料

參考文獻(xiàn)
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱(chēng):
Fibrinogen (Bovine)
目錄號(hào):
HY-125864
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