Thrombin (MW 37kDa) is a Na⁺-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.

在室溫下加入 Thrombin (MW 37kDa)（1 單位/100 μg 蛋白質(zhì)）20 小時(shí)后，p62 就會(huì)從瓊脂糖珠和 GST 標(biāo)簽上裂解下來^[3]。
Thrombin (MW 37kDa) 溶液：溶解于預(yù)冷至 4°C 的 PBS 中。輕輕旋轉(zhuǎn)。將溶液分裝在小份中并置于 -80°C 下保存以保持活性。
裂解緩沖液：PBS（140 mM NaCl、2.7 mM KCl、10 mM Na₂HPO₄、1.8 mM KH₂PO₄）pH 7.3。
洗脫緩沖液：50 mM Tris-HCl，10 mM 還原谷胱甘肽，pH 8.0

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

37000(Average)

9002-04-4

固體

White to off-white

凝血酶

Room temperature in continental US; may vary elsewhere.

• [1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4.  [Content Brief]

  [2]. Krenzlin H, et al. The Importance of Thrombin in Cerebral Injury and Disease. Int J Mol Sci. 2016 Jan 11;17(1).  [Content Brief]

  [3]. Geetha T, et al. p62 serves as a shuttling factor for TrkA interaction with the proteasome. Biochem Biophys Res Commun. 2008 Sep 12;374(1):33-7.  [Content Brief]