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Immunology & Inflammation
NADPH-oxidase inhibitor
-
ROS activator
-
Apoptosis related activator
-
Mitophagy activator
-
Mitochondrial Metabolism modulator
Rotenone
僅限科研使用
Rotenone
別名: Barbasco, Dactinol, Paraderil, Rotenon, Rotocide 中文名稱：魚藤酮
Rotenone是一種植物殺蟲劑，是線粒體電子傳遞mitochondrial electron transport的抑制劑。Rotenone 可抑制 NADH/DB oxidoreductase 和 NADH oxidase，對應(yīng)的IC50值分別為28.8 nM和5.1 nM。Rotenone 可通過增強(qiáng)線粒體活性氧的產(chǎn)生來誘導(dǎo)凋亡。Rotenone 可用于誘導(dǎo)帕金森病動物模型。
Rotenone Chemical Structure
CAS: 83-79-4
產(chǎn)品質(zhì)控
批次：
純度：
99.97%
99.97
Rotenone相關(guān)產(chǎn)品
| 相關(guān)靶點(diǎn) | NOX1 NOX4 NOX2 | 點(diǎn)擊展開 |
|---|---|---|
| 相關(guān)產(chǎn)品 | Setanaxib (GKT137831) DPI (Diphenyleneiodonium chloride) VAS2870 GSK2795039 GLX351322 2-Acetylphenothiazine (ML171) | 點(diǎn)擊展開 |
| 相關(guān)化合物庫 | 激酶抑制劑庫 FDA藥物庫 天然產(chǎn)物庫 已知活性藥物庫-I 高選擇性抑制劑庫 | 點(diǎn)擊展開 |
細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例
| 細(xì)胞系 | 實(shí)驗(yàn)類型 | 給藥濃度 | 孵育時間 | 活性描述 | 文獻(xiàn)信息 |
|---|---|---|---|---|---|
| MG63 | Function assay | 1 uM | 1 hr | Increase in lactate production in MG63 cells at 1 uM after 1 hr by ELISA | 17088489 |
| N548 | Activation of AKT in | 10 uM | 6 hrs | Activation of AKT in DNA-deficient rat striatal neuronal N548 mutant cells at 10 uM after 6 hrs by Western blotting | 17726098 |
| N548 | Cell death rescue assay | 10 uM | 7 days | Cell death rescue in rat striatal neuronal N548 mutant cells analysed every day for 7 days at 10 uM by trypan blue dye-exclusion assay | 17726098 |
| T47D | Function assay | 0.01 to 0.1 uM | 72 hrs | Reduction in ATP level in human T47D cells at 0.01 to 0.1 uM after 72 hrs by HPLC-UV analysis | 21875114 |
| T47D | Function assay | 0.1 to 1 uM | 30 mins | Inhibition of mTOR in human T47D cells assessed as eIF2alpha hyperphosphorylation on Ser 51 at 0.1 to 1 uM after 30 mins by Western blotting | 21875114 |
| T47D | Function assay | 0.1 to 1 uM | 30 mins | Inhibition of mTOR in human T47D cells assessed as eEF2 hyperphosphorylation on Ser 56 at 0.1 to 1 uM after 30 mins by Western blotting | 21875114 |
| MDA-MB-231 | Function assay | 0.1 to 1 uM | 30 mins | Inhibition of mTOR in human MDA-MB-231 cells assessed as eIF2alpha hyperphosphorylation on Ser 51 at 0.1 to 1 uM after 30 mins by Western blotting | 21875114 |
| MDA-MB-231 | Function assay | 0.1 to 1 uM | 30 mins | Inhibition of mTOR in human MDA-MB-231 cells assessed as eEF2 hyperphosphorylation on Ser 56 at 0.1 to 1 uM after 30 mins by Western blotting | 21875114 |
| MDA-MB-231 | Function assay | 0.01 to 0.1 uM | 48 hrs | Reduction in ATP level in human MDA-MB-231 cells at 0.01 to 0.1 uM after 48 hrs by HPLC-UV analysis | 21875114 |
| N548 | Activation of ERK in | 10 uM | Activation of ERK in rat striatal neuronal N548 mutant cells at 10 uM by Western blotting | 17726098 | |
| N548 | Activation of AKT in | 10 uM | Activation of AKT in rat striatal neuronal N548 mutant cells at 10 uM by Western blotting | 17726098 | |
| N548 | Activation of ERK in | 50 uM | Activation of ERK in rat striatal neuronal N548 mutant cells at 50 uM by Western blotting | 17726098 | |
| N548 | Activation of AKT in | 50 uM | Activation of AKT in rat striatal neuronal N548 mutant cells at 50 uM by Western blotting | 17726098 | |
| N548 | Cell death rescue assay | 10 uM | Cell death rescue in DNA-deficient rat striatal neuronal N548 mutant cells at 10 uM | 17726098 | |
| T47D | Function assay | 0.001 to 0.1 uM | Inhibition of cellular respiration in human T47D cells assessed as rate of oxygen consumption at 0.001 to 0.1 uM | 21875114 | |
| TA3/Ha | Function assay | 12 uM | Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 12 uM by spectrofluorometer analysis | 24568614 | |
| U937 | Antiproliferative assay | 72 hrs | Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay, IC50=0.07μM | 17158054 | |
| U937 | Antiproliferative assay | 48 hrs | Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay, IC50=0.2μM | 17158054 | |
| U937 | Antiproliferative assay | 24 hrs | Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay, IC50=13μM | 17158054 | |
| HepG2 | Antitumor assay | 24 hrs | Antitumor activity against human HepG2 cells after 24 hrs by MTT assay, ED50=15μM | 17482824 | |
| A549 | Antitumor assay | 24 hrs | Antitumor activity against human A549 cells after 24 hrs by MTT assay, ED50=26μM | 17482824 | |
| U373 MG | Antiproliferative assay | 72 hrs | Antiproliferative activity against human U373 MG cells after 72 hrs | 18513974 | |
| T47D | Function assay | 48 hrs | Reduction in ATP level in human T47D cells after 48 hrs by HPLC-UV analysis | 21875114 | |
| 697 | Cytotoxicity assay | 72 hrs | Cytotoxicity against human 697 cells after 72 hrs by MTS assay, IC50=0.3μM | 23895019 | |
| 697 | Cytotoxicity assay | 48 hrs | Cytotoxicity against human 697 cells after 48 hrs by MTS assay, IC50=0.3μM | 23895019 | |
| HT-29 | Cytotoxicity assay | 72 hrs | Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay, IC50=0.3μM | 23895019 | |
| Raji | Cytotoxicity assay | 72 hrs | Cytotoxicity against human Raji cells after 72 hrs by MTS assay, IC50=1.1μM | 23895019 | |
| Raji | Cytotoxicity assay | 48 hrs | Cytotoxicity against human Raji cells after 48 hrs by MTS assay, IC50=4.2μM | 23895019 | |
| HepG2 | Cytotoxicity assay | 24 hrs | Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay, IC50=1.52μM | 30613327 | |
| HEK293 | Function assay | Effect on cofilin1 expressed in HEK293 cells assessed as effect on cofilin1; Limk2 interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay | 16680159 | ||
| HEK293 | Function assay | Inhibition of of c-Jun expressed in HEK293 cells assessed as induction of protein interaction in presence of camptothecin with Pin1 by EYFP based reporter gene assay | 16680159 | ||
| HEK293 | Function assay | Effect on p53 expressed in HEK293 cells assessed as effect on p53-p53 interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay | 16680159 | ||
| HEK293 | Function assay | Effect on Cdc2 expressed in HEK293 cells assessed as effect on Cdc2:Cdc25A interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay | 16680159 | ||
| HEK293 | Function assay | Effect on Cdc2 expressed in HEK293 cells assessed as effect on Cdc2:Cdc25C interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay | 16680159 | ||
| HT1080 | Function assay | Inhibition of proMMP2 production in human HT1080 cells by gelatin zymography analysis | 17158054 | ||
| HT1080 | Function assay | Inhibition of proMMP9 production in human HT1080 cells by gelatin zymography analysis | 17158054 | ||
| neural precursor cells | Function assay | Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay | 17417631 | ||
| N548 | Cell death rescue assay | Cell death rescue in rat striatal neuron N548 mutant cells, EC50=0.5μM | 17726098 | ||
| ASH neuronal cells | Cell death rescue assay | Cell death rescue of ASH neuronal cells in Caenorhabditis elegans Huntington's disease model, EC50=2μM | 17726098 | ||
| neuronal cells | Cell death rescue assay | Cell death rescue of neuronal cells in drosophila Huntington's disease model, EC50=10μM | 17726098 | ||
| N548 | Toxicity assay | Toxicity in rat striatal neuronal N548 mutant cells, TC50=40μM | 17726098 | ||
| N548 | Cell death rescue assay | Suppression of cell death in rat striatal neuronal N548 mutant cells assessed as suppression of caspase 7 activation by Western blotting | 17726098 | ||
| N548 | Cell death rescue assay | Cell death rescue in rat striatal neuronal N548 mutant cells by calcein acetoxymethyl ester assay | 17726098 | ||
| N548 | Cell death rescue assay | Suppression of cell death in rat striatal neuronal N548 mutant cells assessed as suppression of caspase 3 activation by Western blotting | 17726098 | ||
| Hepa-1c1c7 | Cytotoxicity assay | Cytotoxicity against mouse Hepa-1c1c7 cells, IC50=0.7μM | 23895019 | ||
| Hepa1c1c7 | Function assay | Induction of quinone reductase in mouse Hepa1c1c7 cells, CD=0.8μM | 23895019 | ||
| L5178Y | Cytotoxicity assay | Cytotoxicity against mouse L5178Y cells by MTT assay, IC50=0.3μM | 28075580 | ||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells | 29435139 | ||
| DAOY | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells | 29435139 | ||
| SJ-GBM2 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells | 29435139 | ||
| A673 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells | 29435139 | ||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells | 29435139 | ||
| BT-37 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells | 29435139 | ||
| NB-EBc1 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells | 29435139 | ||
| Saos-2 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells | 29435139 | ||
| LAN-5 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells | 29435139 | ||
| BT-12 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells | 29435139 | ||
| OHS-50 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells | 29435139 | ||
| Rh41 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells | 29435139 | ||
| A673 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for A673 cells) | 29435139 | ||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells | 29435139 | ||
| BT-12 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-12 cells | 29435139 | ||
| LAN-5 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for LAN-5 cells | 29435139 | ||
| NB-EBc1 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB-EBc1 cells | 29435139 | ||
| BT-37 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-37 cells | 29435139 | ||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells | 29435139 | ||
| MG 63 (6-TG R) | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for MG 63 (6-TG R) cells | 29435139 | ||
| fibroblast cells | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for control Hh wild type fibroblast cells | 29435139 | ||
| OHS-50 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for OHS-50 cells | 29435139 | ||
| DLD1 | Function assay | Inhibition of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as reduction in oxygen consumption rate by seahorse XFe96 analyser based assay | 31774672 | ||
| 點(diǎn)擊查看更多細(xì)胞系數(shù)據(jù) | |||||
生物活性
| 產(chǎn)品描述 | Rotenone是一種植物殺蟲劑，是線粒體電子傳遞mitochondrial electron transport的抑制劑。Rotenone 可抑制 NADH/DB oxidoreductase 和 NADH oxidase，對應(yīng)的IC50值分別為28.8 nM和5.1 nM。Rotenone 可通過增強(qiáng)線粒體活性氧的產(chǎn)生來誘導(dǎo)凋亡。Rotenone 可用于誘導(dǎo)帕金森病動物模型。 |
|---|
化學(xué)信息&溶解度
| 分子量 | 394.42 | 分子式 | C23H22O6 |
| CAS號 | 83-79-4 | SDF | Download Rotenone SDF |
| 儲存條件(自收到貨起) | |||
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體外溶解度 |
DMSO : 40 mg/mL ( (101.41 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO) Water : Insoluble Ethanol : Insoluble |
摩爾濃度計(jì)算器 |
|
體內(nèi)溶解度 現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑 |
動物體內(nèi)配方計(jì)算器 | ||||
實(shí)驗(yàn)計(jì)算
摩爾濃度計(jì)算器
動物體內(nèi)配方計(jì)算器（澄清溶液）
第一步：請輸入基本實(shí)驗(yàn)信息（考慮到實(shí)驗(yàn)過程中的損耗，建議多配一只動物的藥量）
mg/kg
g
μL
只
第二步：請輸入動物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
計(jì)算結(jié)果：
工作液濃度： mg/ml；
DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；
體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH2O，混勻澄清。
體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。
注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。
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