7-Ethyl-10-hydroxycamptothecin

Catalog No.

N2133

TOPO I inhibitor

Grouped product items
Size	Price	Stock	Qty
10mM (in 1mL DMSO)	$61.00	In stock
20mg	$55.00	In stock

For scientific research use only and should not be used for diagnostic or medical purposes.

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Background

Extracted?from?Camptotheca acuminata Decne. fruit, leaf, branch;Store?the?product?in?sealed,?cool?and?dry?condition

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	392.4
Cas No.	86639-52-3
Formula	C₂₂H₂₀N₂O₅
Solubility	insoluble in H2O; insoluble in EtOH; ≥11.15 mg/mL in DMSO
SDF	Download SDF
Canonical SMILES	O[C@@]1(CC)C2=C(COC1=O)C(N(CC3=C4N=C(C=C5)C(C=C5O)=C3CC)C4=C2)=O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Cell experiment:[1]
Cell lines	The parental human colon cancer cell line KM12C and its two highly metastatic derivatives KM12SM and KM12L4a
Reaction Conditions	2.5 μg/ml 7-ethyl-10-hydroxycamptothecin for 4, 24 or 48 h incubation
Applications	The exposure of the cells to 7-ethyl-10-hydroxycamptothecin induced S-phase and G2 arrest and the KM12L4a cells had the highest response in a time-dependent manner. The rates of apoptosis in the KM12SM and KM12L4a cell lines were increased time-dependently, though there was no such change in the KM12C cells. In conclusion, the effect of 7-ethyl-10-hydroxycamptothecin on the colon cancer cell lines was mediated via conducting S-phase and G2 arrest and apoptosis. This effect was found in the cell lines with higher metastatic potentials, indicating that 7-ethyl-10-hydroxycamptothecin can be used to treat advanced colon cancers.
Note	The technical data provided above is for reference only.
	References: 1. Wallin A, Svanvik J, Holmlund B, et al. Anticancer effect of SN-38 on colon cancer cell lines with different metastatic potential. Oncology Reports, 2008, 19(6): 1493-1498.

Description	7-Ethyl-10-hydroxycamptothecin is an inhibitor of DNA topoisomerase I with IC50 value of 77 nM.
Targets	DNA topoisomerase I
IC50	77 nM

Purity = 99.48%