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- Isoprenaline HCl
Isoprenaline HCl
Isoprenaline HCl is an agonist of β-adrenergic receptor [1].
The adrenergic receptors are a class of G protein-coupled receptors that are targets of norepinephrine and epinephrine. When reacting with epinephrine, β-adrenergic receptor causes vasodilation.
In human umbilical vein endothelial cells (HUVECs), isoprenaline (100 nM) significantly increased the expression of connexins Cx43 and also increased Cx40 and Cx37. Also, isoprenaline increased the number of coupling cells. Isoprenaline enhanced the formation of branches and complex tube networks [2].
In the isolated field stimulated rat vas deferens, isoprenaline inhibited contractions with EC50 value of 45.6 nM. In tissues in which β 2-adrenoreceptors were maximally blocked by timolol, isoprenaline inhibited contractility with EC50 value of 1.5 μM [1]. In CFW mice, IPR caused the maximal proliferative response of bone marrow cells and increased erythropoietic activity. These results suggested that isoprenaline activated beta-adrenergic receptor, which then increased the proliferative and differentiation activities of hemopoietic stem cells [3].
References:
[1].? Lotti VJ, Cerino D, Kling P. Characterization of the adrenoreceptor activities of isoprenaline in the field stimulated rat vas deferens: selective supersensitivity to beta 2-mediated responses following reserpine treatment. J Auton Pharmacol, 1982, 2(3): 169-174.
[2].? Dhein S, Gaertner C, Georgieff C, et al. Effects of isoprenaline on endothelial connexins and angiogenesis in a human endothelial cell culture system. Naunyn Schmiedebergs Arch Pharmacol, 2015, 388(1): 101-108.
[3].? Lipski S. Effect of beta-adrenergic stimulation by isoprenaline on proliferation and differentation of mouse bone marrow cells in vivo. Pol J Pharmacol Pharm, 1980, 32(3): 281-287.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 247.72 |
| Cas No. | 51-30-9 |
| Formula | C11H17NO3·HCl |
| Solubility | ≥12.39 mg/mL in DMSO; ≥16.6 mg/mL in EtOH with gentle warming and ultrasonic; ≥50.2 mg/mL in H2O with gentle warming |
| Chemical Name | 4-[1-hydroxy-2-(propan-2-ylamino)ethyl]benzene-1,2-diol;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CC(C)NCC(C1=CC(=C(C=C1)O)O)O.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Human umbilical vein endothelial cells (HUVECs) |
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Reaction Conditions |
100 nmol/L isoprenaline for 20 h incubation |
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Applications |
Isoprenaline resulted in significantly enhanced expression of endothelial Cx43 and to a lower degree of Cx40 and Cx37. The number of coupling cells was significantly increased. Regarding angiogenesis, isoprenaline led to significantly enhanced formation of branches and a higher complexity of the tube networks with more branches/length. |
| Animal experiment:[2] | |
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Animal models |
Male albino rats of the Sprague-Dawley strain, 225 ~ 325 g |
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Dosage form |
0.33 mg/kg Administered by subcutaneous injection |
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Applications |
A subcutaneous injection of 0.33 mg/kg isoproterenol decreased blood pressure and increased water intake in nephrectomized rats. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Dhein S, Gaertner C, Georgieff C, et al. Effects of isoprenaline on endothelial connexins and angiogenesis in a human endothelial cell culture system. Naunyn-Schmiedeberg's Archives of Pharmacology, 2015, 388(1): 101-108. 2. Hosutt JA, Rowland N, Stricker EM. Hypotension and thirst in rats after isoproterenol treatment. Physiology & Behavior, 1978, 21(4): 593-598. |
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